The technical development and application prospects of CRISPR/Cas9 in laboratory medicine
10.3760/cma.j.issn.1009-9158.2019.05.012
- VernacularTitle:CRISPR/Cas9在医学检验中的研究进展及应用
- Author:
Xiaojing YIN
1
;
Jiabo HU
;
Chongxu HAN
;
Yunxia WANG
;
Weiling FU
;
Yang ZHANG
Author Information
1. 江苏大学医学院检验医学系
- Keywords:
CRISPR-Associated Protein 9;
CRISPR-cas systems;
Gene editing;
Nucleic acid amplification techniques;
Bacterial typing techniques
- From:
Chinese Journal of Laboratory Medicine
2019;42(5):381-385
- CountryChina
- Language:Chinese
-
Abstract:
Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9),a cluster of regularly spaced short palindromic repeats,is a natural immune defense system for bacteria and archaea to identify themselves and exogenous invading DNA fragments,protecting them from viruses.In recent years,CRISPR/Cas9 has become a revolutionary gene editing tool.Its specific targeted spot-cutting ability also plays an important role in nucleic acid detection,bacterial typing,etc.,and has shown great application potential in the field of medical testing.Based on the latest researches,this paper reviews the progress of CRISPR/Cas9 application in the new techniques of nucleic acid detection,pathogen typing and bacterial evolution in laboratory medicine,and also summarizes the application prospect of CRISPR technology in the field of laboratory medicine.
