Correlation between expression of Brf1 and prognosis in non-small cell lung cancer
10.3760/cma.j.issn.1001-4497.2019.08.010
- VernacularTitle:非小细胞肺癌中Brf1表达与预后的相关性
- Author:
Hao LYU
1
;
Yunfeng YI
;
Zhiming SONG
;
Jianming CHEN
;
Changjie JIAO
;
Songlin CHEN
;
Shuping ZHONG
Author Information
1. 南昌大学研究生院医学部 330006
- Keywords:
Non-small cell lung cancer;
Transcription factor Ⅱ B-related factor;
RNA polymerase Ⅲ-dependent genes;
Prognosis
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2019;35(8):489-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression of transcription factor ⅡB-related factor 1 (Brf1) and the prognosis of non-small cell lung cancer (NSCLC).Methods Collected 96 cases of NSCLC Surgical specimens and clinical data of patients from January 2013 to August 2015 in our hospital.First of all,we compared the expression of Brf1 in NSCLC tissues and adjacent lung tissues by Western blot and RT-qPCR.Then,Immunohistochemistry was used to detect the expression of Brf1 in NSCLC tissues,and analysis of the relationship between Brf1 expression level and clinical case characteristics.Survival curves were plotted using the Kaplan-Meier method and Log-rank test and multivariate Coxv regression analysis were performed.Results Western blot and RT-qPCR results showed that the expression of Brf1 in NSCLC tissues was significantly higher than that in adjacent lung tissues (P ＜0.01).The positive expression rate of Brf1 in 96 cases of NSCLC was 72.9％.The Brf1 expression level was higher in the poorly differentiated group than in the moderately-highly differentiated group(Mean Rank 62.33 ＞ 43.89,Z =-2.914,P =0.004),and the lymph node metastasis group was higher than the non-metastasis group(Mean Rank 60.34 ＞ 42.58,Z =-3.055,P =0.002),which was independent of patient gender,age,smoking status,tumor size,TNM stage,and pathological type (P ＞0.05).Single-factor survival analysis by Log-rank test showed that the survival rate of Brf1 positive expression group was lower than that of the negative group (x2 =7.560,P ＜0.01).Multivariate analysis of Cox regression model found that Brf1 positive expression (HR =2.043,95％ CI:1.082-3.860) was an independent observational index that affects the prognosis of patients with NSCLC.Conclusion Brf1 is overexpressed in NSCLC tissues,and Brf1 negative expression has a good clinical prognosis,suggesting that Brf1 may be one of the indicators of malignant degree and prognosis of NSCLC.
