The Comparison of the Effect of Cigarette and Stop Smoking-aiding Cigarette on Release of IL-6 from Bronchial Epithelial Cell.
10.4046/trd.2005.59.5.530
- Author:
Myoung Chan KIM
1
;
Jeil JUNG
;
Jong Hoon JUNG
;
Hak Ryul KIM
;
Sei Hoon YANG
;
Eun Taik JEONG
;
Hui Jung KIM
Author Information
1. Department of Internal Medicine, Seoul Adventist Hospital, Korea.
- Publication Type:Original Article
- Keywords:
Cigarette;
stop smoking-aiding cigarette;
IL-6
- MeSH:
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells*;
Inflammation;
Interleukin-6*;
Pulmonary Disease, Chronic Obstructive;
RNA, Messenger;
Smoke;
Smoking;
Smoking Cessation;
Tobacco Products*
- From:Tuberculosis and Respiratory Diseases
2005;59(5):530-535
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND AIMS: Cigarette smoking induces an inflammatory response in the airways, which may play a key role in the pathogenesis of chronic obstructive pulmonary disease. Interleukin-6 (IL-6) is one of the cytokines that plays an important role in inducing bronchial inflammation. The aim of this study was to determine if the level of the pro-inflammatory cytokine, Interleukin-6 , is increased when the bronchial epithelial cells are exposed to a cigarette smoke extract (CSE) and an extract from stop smoking-aiding cigarettes, and examined the safety of these commercially available stop smoking-aiding cigarettes. METHOD: Bronchial epithelial cells were exposed to CSE from cigarette and stop smoking-aiding cigarettes for 24 hours. ELISA was used to measure the IL-6 levels in the supernatant from each condition. The IL-6 mRNA levels were measured by Taqman Real time RT-PCR. N-acetyl-L-cysteine(NAC) was added to each condition to determine if NAC can inhibit the release of IL-6 from the bronchial epithelial cells when they are exposed to CSE from cigarette and stop smoking-aiding cigarettes. RESULT: When bronchial epithelial cells were exposed to a CSE from cigarettes and stop smoking- aiding cigarettes, each type of CSE stimulated IL-6 production from the bronchial epithelial cells. The IL-6 mRNA level in the Bronchial epithelial cells was also elevated and NAC was found to inhibit the release of IL-6 from bronchial epithelial cells when they were exposed to the CSE from cigarettes and stop smoking-aiding cigarettes. CONCLUSION: Commercially available stop smoking-aiding cigarette can induce bronchial inflammation and can be harmful to smokers. Therefore, the safety of these cigarettes for smoking cessation should be evaluated.
