Molecular detection and sequence analysis of enteroviruses isolated from a suspected outbreak of aseptic meningitis
10.3760/cma.j.issn.0254-5101.2019.08.006
- VernacularTitle:一起疑似无菌性脑膜炎暴发疫情样本中肠道病毒的分子检测与序列分析
- Author:
Guangming ZHANG
1
;
Jianyi MAI
;
Zejun WANG
;
Shengli MENG
;
Zhifeng PANG
;
Meng'an CHEN
;
Shuo SHEN
Author Information
1. 金华市疾病预防控制中心传染病防制科 321002
- Keywords:
Aseptic meningitis;
Enterovirus;
Coxsackievirus A10;
Echovirus 6
- From:Chinese Journal of Microbiology and Immunology
2019;39(8):596-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect and analyze enteroviruses causing suspected aseptic meningitis in a kindergarten in Jinhua City, Zhejiang Province. Methods Viral RNA was extracted from samples and cDNA was prepared by reverse transcription. PCR was performed to amplify the partial sequences of the 5′-untranslated region ( UTR) and VP1 gene of enteroviruses. Serotypes of the viruses were determined by com-paring the homology between the partial sequences of VP1 gene. Phylogenetic tree of the partial VP1 se-quences was constructed using MEGA6. Results This study included seven patients and twenty-six asymp-tomatic students. Coxsakievirus A10 (CV-A10) was detected in 48. 5％ of the students and echovirus 6 (Echo 6) in 21. 2％. Besides, 12. 1％ of the students might be co-infected by the two viruses. Among the seven patients, six were infected by CV-A10 and the other one might have co-infection. According to the phylogenetic analysis, CV-A10 strains detected in this study were closely related to those isolated in China in recent years, including the strains isolated in Xiamen in 2015 and Yunnan in 2017, while the Echo 6 strains were phylogenetically related to those isolated in Yunnan, Guangzhou and Shandong in 2014. Conclusions CV-A10 and Echo 6 were detected in the cases with suspected aseptic meningitis and had close phylogenetic relationships to the strains appeared in China in recent years.
