Analysis of genomic characteristics and virulence factors of emetic-type Bacillus cereus strains isola-ted in Hangzhou
10.3760/cma.j.issn.0254-5101.2019.08.002
- VernacularTitle:杭州地区催吐型蜡样芽孢杆菌基因组特征与毒力因子分析
- Author:
Xiaodong LIU
1
;
Haoqiu WANG
;
Xiuqin LOU
;
Wei ZHENG
;
Zhibei ZHENG
;
Tao LIU
;
Wei ZHANG
;
Qi CHEN
;
Shi CHENG
;
Hua YU
Author Information
1. 杭州市疾病预防控制中心微生物检验科 310021
- Keywords:
Bacillus cereus;
Genome sequencing;
Virulence factor;
Genomic analysis
- From:
Chinese Journal of Microbiology and Immunology
2019;39(8):572-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genomic characteristics and virulence factors of emetic-type Bacillus cereus strains isolated from food in Hangzhou for better understanding their pathogenic potential. Methods Real-time PCR was performed to detect the ces gene cluster ( cereulide) in 132 Bacillus cereus strains isolated from food from 2015 to 2017. Genomes of cereulide-positive strains were sequenced using Illumina MiSeq sequencing platform. Genome annotation, virulence factor detection, comparative and evolu-tionary analysis were performed after the sequences of genomes were assembled. Results Twelve strains (9. 09％) carried the ces gene. Their genome sizes ranged from 5. 35 to 5. 75 Mb and GC contents from 35. 25 to 35. 43 mol％. All of them harbored the full cereulide biosynthesis gene cluster, nonhemolytic ente-rotoxin ( NHE)-encoding gene cluster ( nheA, nheB and nheC) and hemolysinⅢ( hlyⅢ) . The average nu-cleotide identity ( ANI ) between the 12 isolates and the reference strain NC7401 ( Accession number:AP007209) was over 99. 35％. Phylogenetic analysis demonstrated these strains were clustered into the same branch with local clinical isolates and the emetic-type Bacillus cereus strains of NC7401 and AH187. Con-clusions The genomic sequences of the emetic-type Bacillus cereus strains isolated from food in Hangzhou area were highly similar to that of the reference strain NC7401. Results of the genomic analysis suggested that these isolates carried many virulence factors that were related to pathogenicity.
