Adjuvant activity of alum adjuvant and MF59 for HIV-1 multi-epitope protein MEP1
10.3760/cma.j.issn.0254-5101.2019.07.003
- VernacularTitle:铝佐剂和MF59对HIV-1多表位蛋白MEP1佐剂活性的比较研究
- Author:
Yuepeng WANG
1
;
Qiao LI
;
Qing ZHU
;
Yi YANG
;
Yan GUO
;
Yusen ZHOU
;
Zhihua KOU
Author Information
1. 安徽医科大学北京微生物流行病研究所
- Keywords:
MF59;
Aluminum adjuvant;
Protein vaccine;
Cytokine;
Antibody
- From:Chinese Journal of Microbiology and Immunology
2019;39(7):499-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the adjuvant activity of oil-in-water emulsion MF59 and aluminum hydroxide on immune responses to HIV-1 multi-epitope protein MEP1 in BALB/c mice. Methods HIV-1 multi-epitope protein MEP1 with MF59 or aluminum was prepared to intramuscularly vaccinate female BALB/c mice for three times. Serum and splenocytes were isolated from the mice 10 d after the first and last vaccination. Specific anti-MEP1 antibodies and the subclasses in serum were detected by ELISA. The num-bers of splenic lymphocytes secreting IFN-γ and IL-4 were measured by ELISPOT. Differences in humoral and cellular immune responses induced by the two different adjuvants were compared. Results After a sin-gle-dose immunization, aluminum adjuvant promoted early humoral and cellular immune responses to MEP1 compared with MF59. After the three-dose immunization, both aluminum adjuvant and MF59 promoted MEP1 to induce Th2-biased humoral immune response, while MF59 also enhanced a balanced Th1/Th2 cel-lular immune response. Conclusions Aluminum adjuvant was a more suitable adjuvant than MF59 for HIV-1 multi-epitope protein MEP1.
