Activities of polysaccharides in combination with aluminum adjuvant in varicella-zoster subunit vaccines
10.3760/cma.j.issn.0254-5101.2019.06.008
- VernacularTitle:植物源多糖联合铝佐剂在水痘-带状疱疹亚单位疫苗中的免疫佐剂活性研究
- Author:
Meifeng NIE
1
;
Yike LI
;
Xiaofen HUANG
;
Tong CHENG
;
Qinjian ZHAO
Author Information
1. 厦门大学公共卫生学院 分子疫苗学和分子诊断学国家重点实验室 国家传染病诊断试剂与疫苗工程技术研究中心 361102
- Keywords:
Varicella-zoster virus;
Vaccine;
Plant-derived polysaccharide;
Aluminum adjuvant;
Immunostimulatory effect
- From:
Chinese Journal of Microbiology and Immunology
2019;39(6):454-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immunostimulatory effects of polysaccharides, including Lycium barbarum polysaccharides ( LBP) , Angelica polysaccharides ( AP) , Licorice polysaccharides ( LP) and Inulin polysaccharides ( IP) , used alone or in combination with aluminum adjuvant on the recombinant protein of varicella-zoster virus (VZV) glycoprotein (gE) as an immunogen in mice. Methods BALB/c mice were randomly divided into 11 groups with five in each group. Intramuscular injection was given at Days 0 and 14. Serum samples were collected at weeks 0, 2, 3, 4 and detected for total anti-gE IgG titers and an-tibody subtypes by indirect ELISA. The mice were sacrificed at week 4 to collect spleen lymphocytes aseptic-ally. CCK-8 method was used to evaluate the proliferation of spleen lymphocytes. The levels of IFN-γ and IL-4 were measured by ELISA. The percentages of CD3+CD4+ and CD3+CD8+ T cell subsets were deter-mined by flow cytometry. Results The four plant-derived polysaccharides in combination with aluminum adjuvant showed good adjuvant activities. LP combined with aluminum adjuvant effectively increased the titer of IgG2, promote the proliferation of splenocytes and enhanced the secretion of IFN-γ and IL-4. Further-more, it also induced CD3+CD4+ and CD3+CD8+ T cell proliferation in vivo. Conclusions The four plant-derived polysaccharides in combination with aluminum adjuvant could all enhance antibody production. LP combined with aluminum adjuvant could induce cell-mediated immune responses, suggesting that it might be a promising adjuvant for varicella-zoster subunit vaccines.
