Screening, preparation and identification of a broad-spectrum monoclonal antibody against Strepto-coccus pneumoniae surface protein A
10.3760/cma.j.issn.0254-5101.2019.05.007
- VernacularTitle:一株广谱抗肺炎链球菌表面蛋白A单克隆抗体的筛选、制备及鉴定
- Author:
Xiaonan GUO
1
;
Xiangyu MENG
;
Bo LI
;
Haoxuan GU
;
Lin SUN
;
Yongge WU
;
Tiejun GU
Author Information
1. 吉林大学艾滋病疫苗国家工程实验室
- Keywords:
Streptococcus pneumoniae;
Streptococcus pneumoniae surface protein A ( PspA);
Mon-oclonal antibody
- From:
Chinese Journal of Microbiology and Immunology
2019;39(5):365-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and prepare a broad-spectrum monoclonal antibody against Strep-tococcus pneumoniae ( S. pneumoniae) surface protein A ( PspA) and to evaluate its potential in clinical prac-tice. Methods Hybridoma cells were screened and inoculated into the abdominal cavities of BALB/c mice to prepare antibodies in ascites. Monoclonal antibodies were obtained by ammonium sulfate precipitation and protein A affinity chromatography and then identified by SDS-PAGE and Western blot. Their specificity, iso-forms and killing activities in vitro were analyzed. Results A broad-spectrum monoclonal antibody that rec-ognized PspA subclasses 2, 3 and 4 was obtained. Its in vitro killing rate against S. pneumoniae reached 40. 3％. Conclusions A broad-spectrum monoclonal antibody that could specifically bind to PspA was suc-cessfully prepared with a strong in vitro killing activity. This study provided reference for clinical diagnosis of S. pneumoniae-related diseases, quality assessment of S. pneumoniae vaccines and further research on mono-clonal antibody therapeutics.
