Effects of Fomes officinalis polysaccharide and its purified constituent on mitogen-activated protein kina-ses (MAPK) signaling pathway in RAW264. 7 macrophages
10.3760/cma.j.issn.0254-5101.2019.05.003
- VernacularTitle:阿里红多糖及其组分对RAW264.7巨噬细胞中MAPK信号途径的影响
- Author:
Yuanyuan CONG
1
;
Munisa DILIXIATI
;
Mirensha YAKUFU
;
Ayijiang HABAIKE
;
Palida ABULIZI
Author Information
1. 新疆医科大学药学院
- Keywords:
Fomes officinalis polysaccharide;
RAW264. 7 macrophage;
Mitogen-activated protein ki-nases ( MAPK);
Tumor necrosis factor-α ( TNF-α)
- From:Chinese Journal of Microbiology and Immunology
2019;39(5):333-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of crude Fomes officinalis polysaccharide ( FOPS ) and its purified constituent ( FOPS-a) on mitogen-activated protein kinases ( MAPK) signaling pathway and se-cretion of tumor necrosis factor-α (TNF-α) in RAW264. 7 macrophages. Methods RAW264. 7 macrophages were treated with FOPS and FOPS-a respectively at different concentrations (50, 100, 200 μg/ml) for 24 h. RT-qPCR and Western blot methods were respectively used to detect the expression at mRNA level and the phos-phorylated proteins of p38, c-Jun NH2-terminal kinase ( JNK ) and extracellular signal-regulated kinase ( ERK) . Changes in the phosphorylation of these proteins and TNF-α secretion were respectively detected by Western blot and ELISA after treating the macrophages with MAPK-specific inhibitors ( PD98059, SP600125, SB203580). Results Compared with the blank control group, different concentrations of FOPS and FOPS-a could significantly increase the expression at mRNA level and the phosphorylation of p38 and ERK (P<0. 05). Three inhibitors of MAPK could markedly decrease the phosphorylation of ERK1/2 and p38 and TNF-αsecretion that were induced by FOPS and FOPS-a (P<0. 05). Conclusions FOPS and FOPS-a might have immunomodu-latory effects on RAW264. 7 macrophages through activating ERK1/2 and p38 MAPK signal transduction pathways.
