Molecular characterization of immune response signaling molecules induced by transfection of cox-sackievirus B2 structural proteins into epithelial cells
10.3760/cma.j.issn.0254-5101.2019.05.001
- VernacularTitle:柯萨奇病毒B组2型(CVB2)病毒蛋白转染上皮细胞后诱导的免疫应答信号 分子的特征分析
- Author:
Tangwei MOU
1
;
Huaye WU
;
Lei LIU
;
Jianbin WANG
;
Ying ZHANG
;
Qihan LI
Author Information
1. 中国医学科学院
- Keywords:
Coxsackievirus B2;
Structural protein;
Immune response
- From:Chinese Journal of Microbiology and Immunology
2019;39(5):321-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular characteristics of immune response signaling molecules induced by transfection of coxsackievirus B2 ( CVB2 ) structural proteins into epithelial cells. Methods Recombinant eukaryotic expression plasmids containing the coding regions of CVB2 structural proteins VP1-VP4 were constructed and then transfected into 16HBE cells. Culture supernatants and cell ly-sates of the transfected 16HBE cells were collected. Expression of signaling molecules involved in innate im-mune responses in transfected 16HBE cells at mRNA level was detected by RT-Q-PCR. The proliferation of T cells co-cultured with culture supernatants and cell lysates of the transfected 16HBE cells was analyzed by ELISPOT. Results Expression of innate immunity-related signaling molecules such as TGF-β-activated ki-nase ( TAK) , NF-κB-inducing kinase ( NIK) , IκB kinase α ( IKKα) and IFN-β at mRNA level was up-regulated in 16HBE cells transfected with CVB2 structural proteins VP1-VP4. Both culture supernatants and cell lysates of the transfected 16HBE cells enhanced the proliferation of T cells. Conclusions CVB2 struc-tural proteins VP1-VP4 could enhance the expression of innate immunity-related signaling molecules to var-ying degrees and promote the activation of adaptive immunity.
