Value ofmetagenomic next-generation sequencing in the diagnosis and treatment of severe pneumonia after renal transplantation
10.3760/cma.j.issn.0254-1785.2019.06.009
- VernacularTitle:基于宏基因组学的二代测序技术在肾移植术后重症肺炎诊治中的应用
- Author:
Huichang ZHUO
1
;
Jiandong LIN
;
Yanjing YOU
Author Information
1. 福建医科大学附属第一医院重症医学科
- Keywords:
Renal transplantation;
Pneumonia;
Pathogenic microorganism
- From:
Chinese Journal of Organ Transplantation
2019;40(6):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of metagenomic next-generation sequencing (mNGS) in the diagnosis and treatment of severe pneumonia after renal transplantation .Methods A total of 38 patients with severe pneumonia after renal transplantation from October 2017 to December 2018 were selected and divided into experimental group (A ,n= 15)and control group (B ,n= 23) based upon whether mNGS of bronchoalveolar lavage fluid was employed for detecting pathogenic microorganisms .Positive rate ,clinical acceptance rate ,hospitalization time ,hospitalization expenses and 28-day mortality rate of two methods were compared .Results Positive rate and clinical acceptance rate of mNGS were higher in experimental group than those in traditional experimental and control groups ( P < 0 .05 ) . Hospitalization time , hospitalization expenses and 28-day mortality of experimental group were lower than those of control group (P< 0 .05) .No significant inter-group difference existed in 90-day mortality (P> 0 .05) .Conclusions For patients with severe pneumonia after renal transplantation , mNGS of bronchoalveolar lavage fluid can improve positive rate of etiological diagnosis and clinical acceptance rate and reduce hospitalization time , hospitalization expenses and 28-day mortality .
