Inhibition of islet allograft rejection by Qa-1/PD-L1 artificial liposome
10.3760/cma.j.issn.0254-1785.2019.02.003
- VernacularTitle:Qa-1和PD-L1人工脂质体抑制同种小鼠胰岛移植物排斥反应的作用
- Author:
Meng GUO
1
;
Yuanyu ZHAO
;
Hao YIN
;
Jiayong DONG
;
Junsong JI
;
Qi LU
;
Hang YUAN
;
Fei TENG
;
Wenyuan GUO
;
Guoshan DING
Author Information
1. 第二军医大学附属长征医院器官移植科
- Keywords:
Mouse;
Islet transplantation;
Qa-1;
PD-L1;
Graft rejection;
SHP1/2
- From:
Chinese Journal of Organ Transplantation
2019;40(2):72-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Qa-1 and PD-L1 loaded artificial liposomal treatment in allograft rejection and its outcomes .Methods The extracellular domains of Qa-1 and PD-L1 were loaded on liposome surface by streptavidin-biotin system . Mixed lymphocyte reaction (MLR) was performed for measuring Qa-1/PD-L1 liposome biological function .Then liposome was co-transplanted with allo-islets via portal vein .The levels of blood glucose and C-peptide were detected daily after transplantation .Also hepatic lymphocytes after transplantation were isolated for determining the proportion of activated cells and signaling pathway changes .Results Artificial liposome could be easily loaded with biotinylated peptide and its diameter was between 50 to 500 nm . Qa-1/PD-L1 liposome could significantly suppress lymphocyte proliferation , activation and secretion of IFN-γ in MLR by an activation of SHP1/2 and an inhibition of Syk pathway .Qa-1/ PD-L1 liposomes could suppress the activation of hepatic lymphocytes in vivo by activating SHP1/2 ,protecting islet allografts and maintaining a normal level of blood glucose in recipients .Conclusions Qa-1/PD-L1 loaded liposome can effectively suppress allograft rejection and improve the outcomes of islet transplantation .
