Protective effect of ETaR siRNA on renal ischemia-reperfusion injury in rats by changing the immuno-microenvironment of kidney
10.3760/cma.j.issn.0254-1785.2019.02.002
- VernacularTitle:ETaR siRNA改变肾脏免疫微环境减轻大鼠肾缺血再灌注损伤的作用
- Author:
Yichen JIA
1
;
Long ZHENG
;
Long LI
;
Jiawei LI
;
Ming XU
;
Tongyu ZHU
;
Ruiming RONG
Author Information
1. 复旦大学附属中山医院泌尿外科 上海市器官移植重点实验室
- Keywords:
Rats;
Kidney;
Endothelin receptor;
Small interfering RNA;
Immuno-microenvironment;
Reperfusion injury
- From:
Chinese Journal of Organ Transplantation
2019;40(2):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of ETaR siRNA on renal ischemia reperfusion injury (IRI) by changing the immuno-microenvironment in rats .Methods A total of 40 male Sprague-Dawley (SD) rats were randomized into four groups of sham ,IR ,negative siRNA and ETaR siRNA .A renal IRI model was generated by clamping left renal artery .ETaR siRNA was delivered into kidney through renal vein by a retrograde 'hydrodynamic' injection .Blood samples were collected for detecting renal function and kidney tissue harvested for Hematoxylin & Eosin (HE) staining , TdT-mediated dUTP Nick-End Labeling (TUNEL) staining ,polymerase chain reaction (PCR) and Western blot at 48 h post-reperfusion .Results Serum creatinine ,blood urea nitrogen and renal apoptotic cells increased and renal tissue was injured after IR . The changes were inhibited by ETaR siRNA . PCR showed that ETaR siRNA treatment significantly down-regulated the expressions of inflammatory factors TNF-α , IFN-γ and IL-6 and transcription factor NF-κB induced by IR .Conclusions ETaR siRNA can effectively improve the immunomicroenvironment and thereby alleviate renal ischemia reperfusion injury .
