Establishment and troubleshooting of orthotopic mouse liver transplantation model
10.3760/cma.j.issn.0254-1785.2018.07.010
- VernacularTitle:小鼠原位肝移植模型的建立与难点解析
- Author:
Jing LUO
1
;
Jiequn LI
;
Ting LI
;
Zhongzhou SI
;
Haizhi QI
Author Information
1. 中南大学湘雅二医院普外器官移植科
- Keywords:
Orthotopic liver transplantation;
Cuff;
Animal model
- From:
Chinese Journal of Organ Transplantation
2018;39(7):430-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the orthotopic mouse liver transplantation model and cover troubleshooting,in order to provide experimental techniques support for organ transplantation pathology and immunology studies.Methods Male C57BL/6 mice,10-12 weeks,were selected as the allograft donors.Male C3H mice with same age were selected as the allograft recipients.The orthotopic mouse liver transplantation model consisted of 3 stages,including harvesting the donor liver,back-table preparation of the liver graft and transplantation of the donor liver into the recipient.The average time for harvesting the donor livers was (40 ± 8.8) min,(23 ± 4.7) min for preparing the donor livers and (75 ± 9.6) min for transplanting the donor livers into the recipient.Results Seventy pairs of mice were used for the preliminary experiments.For the formal experiments,the allograft transplantation was established on 220 pairs with 90.4％ successful rate.Conclusion It is the skillful and high quality microsurgical technique that is the guarantee of establishing the orthotopic mouse liver transplantation model successfully.
