Clinical application of flow cytometry versus test tube method to detect antibody titer in ABO incompatible kidney transplantation
10.3760/cma.j.issn.0254-1785.2018.07.005
- VernacularTitle:流式细胞术与传统试管法检测血型抗体滴度在ABO血型不相容肾移植中的临床应用
- Author:
Cuili WANG
1
;
Shi FENG
;
Yucheng WANG
;
Hong JIANG
;
Hongfeng HUANG
;
Jianghua CHEN
Author Information
1. 浙江大学医学院附属第一医院肾脏病中心 浙江省肾脏病防治技术研究重点实验室 国家临床重点专科 浙江大学肾脏病研究所 国家中医药管理局三级实验室“肾脏病免疫实验室”
- Keywords:
Flow cytometry;
ABO incompatible kidney transplantation;
ABO blood type titer
- From:
Chinese Journal of Organ Transplantation
2018;39(7):402-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the flow cytometry versus test tube method in detecting antibody titer in ABO blood type,and try to establish a standard method using flow cytometry to provide insight in ABO incompatible kidney transplantation and therapeutics.Methods The ABO blood type titers of anti-IgM-A/B and anti-IgG-A/B in 30 serum samples from renal allograft recipients were measured by flow cytometry.The results were compared with those determined by test tube method.Results The titers by cytometry significantly higher than those by test tube method (P＜0.05).Conclusion The sensitivity of flow cytometry is significantly higher than test tube method,and flow cytometry can precisely monitor the ABO blood titers in renal allograft recipients,which can provide better medical advice in clinical treatment and therapeutics.
