The expression and clinical significance of 5-hmC and TETs in hepatocellular carcinoma
10.3670.cma.j.issn.1007-631X.2019.08.017
- VernacularTitle:5-羟甲基胞嘧啶和TET家族氧合酶在肝细胞癌中的表达及临床意义
- Author:
Man LI
1
;
Xingang ZHOU
;
Bing SHEN
;
Zhiyuan MA
;
Liang ZHANG
;
Liming QI
;
Peng WANG
Author Information
1. 首都医科大学附属北京地坛医院病理科 100015
- Keywords:
Carcinoma,hepatocellular;
5-methylcytosine;
Oxygenases
- From:
Chinese Journal of General Surgery
2019;34(8):708-711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the levels of 5-hydroxymethylcytosine (5-hmC) and teneleven-translocation proteins (TET1/2/3) in diagnosis and prognosis prediction of hepatocellular carcinoma (HCC).Methods The expression of 5-hmC in 130 cases of HCC tissues were detected by immunohistochemical staining.Kaplan-Meier test was used for survival analysis.TET family plays critical role in the conversion of 5-methylcytosine (5-mC) to 5-hmC.The TET levels were detected by using immunohistochemical staining and RT-PCR,the correlation between 5-hmC and TET was analyzed.Results The level of 5-hmC decreased in HCC tissues,as compared with non-tumor tissues,the expression of TET1 was downregulated in HCC.There was significant difference in the expression between low and high grades of HCC tissues (x2 =10.611,P =0.001).Kaplan-Meier survival analysis showed that there was significant difference between the 5-hmC expression level and the survival rate of HCC patients (x2 =4.412,P =0.036).Conclusions In HCC tissues the expression of 5-hmC was specifically downregulated.Low 5-hmC level is significantly correlated with poor differentiation of the tumor and worse overall survival.Decreased expression of TET1 is likely one of the mechanisms underlying 5-hmC loss in HCC.
