The clinical significance of Th17 cells in patients with myelodysplastic syndrome and iron overload
10.3760/cma.j.issn.0578-1426.2019.06.004
- VernacularTitle:辅助性T细胞17在铁过载骨髓增生异常综合征中的临床意义
- Author:
Kangbao LI
1
;
Qingshan LI
;
Yanhua CHENG
;
Zhigang ZHU
;
Weijie ZHONG
;
Qinghua DU
Author Information
1. 广州市第一人民医院老年病科血液、肿瘤病区 510180
- Keywords:
Th17 cells;
Myelodysplastic syndromes;
Interleukin-6;
Interleukin-17;
Iron overload
- From:
Chinese Journal of Internal Medicine
2019;58(6):419-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the significance of Th17 cells in patients with myelodysplastic syndrome (MDS) and iron overload.Methods A total of 77 patients with MDS admitted to Guangzhou First People's Hospital were enrolled from January 2017 to December 2018,who were divided into iron overload group (37 cases) with serum ferritin (SF) over 1000 μg/L and non-ferrous overload group(40 cases).CD4+T cells in peripheral blood (PB) and bone marrow (BM) were sorted by flow cytometry.The ratio of Th17 cells and cells with abnormal karyotype were compared.IL-17 and IL-6 protein and RNA expression were detected by ELISA and quantitative real-time PCR(qRT-PCR).Results The proportions of Th17 cells in PB and BM in iron overload group were significantly higher than those in non-iron overload group [(41.06± 0.96)％ vs.(26.80± 1.21)％;(47.39± 1.60)％ vs.(34.29± 1.03)％;P＜0.01].The Th17 positive cells with abnormal karyotype in iron overload group were more than those in non-iron overload group[(4.96±0.53)％ vs.(3.67±0.12)％ in PB;(10.06±1.67)％ vs.(4.36±0.43)％ in BM;P＜0.01].Similarly,the protein levels as well as mRNA expression of IL-6 and IL-17 in patients with iron overload were significantly higher than those in non-iron overload group (P＜0.01 both in PB and BM).Conclusions As hematopoietic regulators secreted by Th17 cells,the expression of IL-6 and IL-17 in MDS patients with iron overload are elevated.This may predict the influence of these factors to the differentiation of Th 17 cells.
