Effect of ganglioside preconditioning on endoplasmic reticulum stress-dependent apoptosis during spinal cord injury induced by bupivacaine in rats
10.3760∕cma.j.issn.0254-1416.2019.06.012
- VernacularTitle:神经节苷脂预处理对布比卡因诱发大鼠脊髓损伤时内质网应激途径细胞凋亡的影响
- Author:
Qing FENG
1
;
Benquan LIU
;
Jiemei JI
;
Jingchen LIU
Author Information
1. 广西医科大学第一附属医院麻醉科
- Keywords:
Ganglioside;
Bupivacaine;
Endoplasmic reticulum stress;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2019;39(6):684-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ganglioside ( GM-1) preconditioning on endoplas-mic reticulum stress ( ERS)-dependent apoptosis during spinal cord injury induced by bupivacaine in rats. Methods One hundred and eight clean-grade healthy male Sprague-Dawley rats, aged 8 weeks, weighing 250-300 g, were divided into 3 groups ( n=36 each) using a random number table method: control group (group C), bupivacaine group (group B) and GM-1 pretreatment group (group G). In group B, 5％bupivacaine 0. 12 μl/g was intrathecally injected at 1. 5 h intervals, 3 times intotal. In group G, GM-120μl ( 30 mg/kg) was intrathecally injected, and 24 h later bupivacaine was intrathecally injected according to the method previously described in group B. Immediately after intrathecal injection and at 1, 3, 5, 7 and 14 days after intrathecal injection, the maximum percentage of anti-nociceptive effects (％MPE) was detected, the hindlimb motor function score ( BBB score) was evaluated, and spinal cord tissues were har-vested for determination of cell apoptosis ( using TUNEL) and expression of caspase-12 and CCAAT/enhan-cer-binding protein homologous protein ( CHOP ) protein and mRNA ( by Western blot or quantitative real-time polymerase chain reaction) . The apoptosis rate was calculated. Results Compared with group C, the％MPE and apoptosis rate were significantly increased, the BBB score was decreased, and the expression of CHOP and caspase-12 protein and mRNA was up-regulated in group B ( P＜0. 05) . Compared with group B, the ％MPE and apoptosis rate were significantly decreased, the BBB score was increased, and the ex-pression of CHOP and caspase-12 protein and mRNA was down-regulated in group G ( P＜0. 05) . Conclu-sion GM-1 preconditioning reduces bupivacaine-induced spinal cord injury possibly through inhibiting ERS-dependent apoptosis in rats.
