Relationship between sevoflurane-induced cognitive impairment and α1B and α1D adrenoceptors in cerebral cortex of rats
10.3760/cma.j.issn.0254-1416.2019.05.010
- VernacularTitle:七氟醚诱发大鼠认知功能障碍与大脑皮质α1B和α1D肾上腺素能受体的关系
- Author:
Xiangping JIANG
1
;
Kaiyun FANG
;
Xiang HE
;
Shaopeng GANG
;
Jing WEN
;
Yi MA
;
Siyi YU
Author Information
1. 贵州省安顺市人民医院麻醉科 561000
- Keywords:
Anesthetics,inhalation;
Cognition disorders;
Receptors,adrenergic,alpha-1;
Cerebral cortex
- From:
Chinese Journal of Anesthesiology
2019;39(5):548-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relationship between sevoflurane-induced cognitive impairment and α1B adrenoceptors (ADRA1B) and ADRA1D in the cerebral cortex of rats.Methods Forty-eight SPF adult Sprague-Dawley rats (half male,half female),weighing 220-260 g,were divided into control group (C group,n =24) and sevoflurane group (S group,n =24) using a random number table method.Group C and group S inhaled air and 3％ sevoflurane,respectively,for 5 h.Eight rats in each group were sacrificed immediately after anesthesia,and the cerebral cortex was removed.Eight rats in each group were selected on days 1 and 7 after anesthesia and underwent Barnes maze test.The rats were then sacrificed,and the cerebral cortex was removed.The expression of ADRA1B and ADRA1D protein and mRNA in cerebral cortex tissues was detected by Western blot and fluorescent quantitative real-time polymerase chain reaction,respectively.Results Compared with group C,the number of entering incorrect holes was significantly increased at 1 and 7 days after anesthesia,the latency and total distance to enter the target hole were prolonged,and the expression of ADRA1B and ADRA1D protein and mRNA in cerebral cortex was down-regulated immediately after anesthesia and at 1 and 7 days after anesthesia in group S (P＜0.05).Conclusion The mechanism underlying sevoflurane-induced cognitive impairment may be related to the down-regulated expression of ADRA1B and ADRA1D in cerebral cortex of rats.
