Effect of sevoflurane preconditioning on HMGB1∕TLR4∕NF-κB signaling pathway during lung ische-mia-reperfusion in rats
10.3760∕cma.j.issn.0254-1416.2019.04.014
- VernacularTitle:七氟醚预处理对大鼠肺缺血再灌注时HMGB1∕TLR4∕NF-κB信号通路的影响
- Author:
Shuaiguo LYU
1
;
Xihua LU
;
Tingkun LI
;
Changsheng LI
;
Yi ZHOU
;
Yalin SUN
;
Lei WANG
;
Yu BAI
;
Dandan WANG
Author Information
1. 郑州大学附属肿瘤医院麻醉与围术期医学科 450008
- Keywords:
Anesthetics;
inhalation;
Lung;
Reperfusion injury;
High mobility group pro-teins;
Toll-like receptors;
NF-kappaB
- From:
Chinese Journal of Anesthesiology
2019;39(4):436-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane preconditioning on high-mobility group box 1 protein ( HMGB1) ∕Toll-like receptor 4 ( TLR4) ∕nuclear factor kappa B ( NF-κB) signaling pathway during lung ischemia-reperfusion ( I∕R) in rats. Methods Thirty-six clean-grade healthy male Sprague-Dawley rats, aged 8-10 weeks, weighing 200-250 g, were divided into 3 groups ( n=12 each) using a random number table method: sham operation group ( group S) , lung I∕R group ( group I∕R) and sevoflu-rane preconditioning group ( group SP ) . The right pulmonary hilum was only isolated but not ligated in group S. Lung I∕R was induced by clamping the right pulmonary hilum for 60 min followed by 120 min of reperfusion in anesthetized rats in group I∕R. In group SP, 2. 1％ sevoflurane was inhaled for 30 min to per-form sevoflurane preconditioning, and the lung I∕R model was established at 10 min after the end of inhala-tion. The rats were sacrificed at 120 min of reperfusion, and the lungs were removed for examination of the pathological changes which were scored and for determination of wet to dry weight ratio ( W∕D ratio) , con-tent of tumor necrosis factor-alpha ( TNF-α) in lung tissues ( by enzyme-linked immunosorbent assay) and expression of HMGB1, TLR4 and NF-κB protein in lung tissues (by Western blot). Results Compared with group S, the pathological scores, W∕D ratio and content of TNF-α were significantly increased, and the expression of HMGB1, TLR4 and NF-κB was up-regulated in I∕R and SP groups ( P<0. 05) . Compared with group I∕R, the pathological scores, W∕D ratio and content of TNF-αwere significantly decreased, and the expression of HMGB1, TLR4 and NF-κB was down-regulated ( P<0. 05) , and the pathological changes of lung tissues were significantly attenuated in group SP . Conclusion Sevoflurane preconditioning reduces lung I∕R injury probably through inhibiting HMGB1∕TLR4∕NF-κB signaling pathway in rats.
