Relationship between mechanical ventilation-induced apoptosis in hippocampal neurons and mTOR signaling pathway in mice

Lijuan Tang; Ting Chen; Feng Zheng; Chang Chen; Qi Zhong; Zongze Zhang; Mian Peng; Yan-lin Wang

Return

Relationship between mechanical ventilation-induced apoptosis in hippocampal neurons and mTOR signaling pathway in mice

VernacularTitle:机械通气诱发小鼠海马神经元凋亡与mTOR信号通路的关系
Author: Lijuan TANG ¹ ; Ting CHEN ; Feng ZHENG ; Chang CHEN ; Qi ZHONG ; Zongze ZHANG ; Mian PENG ; Yan-Lin WANG
Author Information

1. 武汉大学中南医院麻醉科 430071
Keywords: Respiration; artificial; Apoptosis; Receptor-interacting protein serine-threonine kina-ses; Cognition disorders
From: Chinese Journal of Anesthesiology 2019;39(4):422-424
CountryChina
Language:Chinese
Abstract: Objective To evaluate the relationship between mechanical ventilation-induced apoptosis in hippocampal neurons and mammalian taget of rapamycin ( mTOR) signaling pathway in mice. Methods Fifty healthy male C57BL∕6 mice, aged 8-10 weeks, weighing 20-25 g, were divided into 2 groups ( n=25 each) using a random number table method: control group ( group C ) and mechanical ventilation group ( group V) . The mice breathed spontaneously for 6 h in group C, and the mice were mechanically ventilated for 6 h in group V. Open field test and contextual fear conditioning test were conducted at 1 and 3 days after the end of ventilation. Hippocampal tissues were obtained at 1 day after the end of ventilation for determina-tion of the expression of mTOR, phosphorylated mTOR (p-mTOR), microtubule-associated protein 1 light chain 3Ⅱ( by Western blot) and apoptosis in hippocampal neurons ( by TUNEL) . The p-mTOR∕mTOR ratio and apoptosis index were calculated. Results Compared with group C, the time animals spent in the central square was significantly prolonged, the number of crossing the grid was reduced, the percentage of freezing time was decreased, the expression of microtubule-associated protein 1 light chain 3Ⅱwas up-regulated, and the p-mTOR∕mTOR ratio and apoptosis index were increased in group V ( P<0. 05) . Conclusion The mech-anism by which mechanical ventilation induces apoptosis in hippocampal neurons may be related to activation of mTOR signaling pathway in mice.