Thalamic paraventricular nucleus mediates orexinergic neurons-induced promotion of emergence from general anesthesia in mice: evaluation using optogenetics method
10.3760/cma.j.issn.0254-1416.2019.03.023
- VernacularTitle:丘脑室旁核介导小鼠orexin能神经元的全麻促觉醒效应:采用光遗传学方法评价
- Author:
Yongxin GUO
1
;
Dan WANG
;
Shiyi ZHAO
;
Xinxin ZHANG
;
Lu YIN
;
Juan GUO
;
Huiming LI
;
Hailong DONG
Author Information
1. 空军军医大学西京医院麻醉与围术期医学科
- Keywords:
Orexin;
General anesthesia;
Wake;
Midline thalamic nuclei
- From:
Chinese Journal of Anesthesiology
2019;39(3):343-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate whether the thalamic paraventricular nucleus mediates orexiner-gic ( orexin ) neurons-induced promotion of emergence from general anesthesia by using the optogenetics method in mice. Methods Twenty healthy male Hcrt-cre mice, aged 8-10 weeks, weighing 20-25 g, were divided into 4 groups ( n=5 each) using a random number table method: retrograde labeled viruses channelrhodopsin group ( R group) , anterograde labeled viruses channelrhodopsin group ( A group) , retro-grade labeled viruses control group ( RC group ) , and anterograde labeled viruses control group ( AC group) . The optogenetics technique was used in each group. Anesthesia was induced and maintained through inhaling 1％ isoflurane and pure oxygen 1. 0 L∕min. Electroencephalogram was monitored througout the procedure with the PowerLab monitoring system. The burst suppression ratio ( BSR) was recorded at 1 min before light stimulation and during light stimulation. Results Compared with RC group or the baseline at 1 min before light stimulation, the BSR was significantly decreased during light stimulation in R group ( P<0. 05) . Compared with AC group or the baseline at 1 min before light stimulation, the BSR was signifi-cantly decreased during light stimulation in group A ( P<0. 05) . Conclusion Optogenetics technique ap-plication once again confirms that orexin neurons can promote emergence from general anesthesia through thalamic paraventricular nucleus in mice.
