Effects of melatonin on excitability of pyramidal neurons in prefrontal cortex and the role of MT1R-cAMP-PKA signaling pathway
10.3760/cma.j.issn.0254-1416.2019.02.025
- VernacularTitle:褪黑素对前额叶皮层锥体神经元兴奋性的影响及MT1R-cAMP-PKA信号通路在其中的作用
- Author:
Jinquan WANG
1
;
Yang CHEN
;
Chao ZHANG
;
Jianteng GU
;
Bin YI
;
Kaizhi LU
;
Ming ZHANG
Author Information
1. 陆军军医大学(原第三军医大学)第一附属医院麻醉科
- Keywords:
Melatonin;
Neurons;
Receptors,melation,MT1;
Cyclic AMP;
Protein kinases
- From:
Chinese Journal of Anesthesiology
2019;39(2):221-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of melatonin on the excitability of pyramidal neurons in the prefrontal cortex and the role of melatonin receptor 1 (MT1 R)-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling pathway.Methods Brains were obtained from male SpragueDawley rats between 14 and 21 days after birth.The brain slices of 350-μm thick were prepared and placed in artificial cerebrospinal fluid.The brain slices were divided into 5 groups (n =6 each) using a random number table method:control group (C group),melatonin group (M group),MT1/2R antagonist luzindole plus melatonin group (L+M group),MT2R antagonist 4P-PDOT plus melatonin group (P+M group) and PKA inhibitor Rp-cAMPS plus melatonin group (R+M group).Cells were perfused for 2 min with artificial cerebrospinal fluid in group C.Cells were perfused for 2 min with 1 μmol/L melatonin in group M.Cells were perfused for 2 min with the mixture of 1 μmol/L MT1/2R antagonist luzindole and 1 μmol/L melatonin in group L+M.Cells were perfused for 2 min with the mixture of 1 μmol/L MT2R antagonist 4P-PDOT and 1 μmol/L melatonin in group P+M.In group R+M,1 mmol/L PKA inhibitor Rp-cAMPS was continuously added to the pipette solution,and cells were perfused for 2 min with 1 μmol/L melatonin.The whole-cell patch-clamp technique was used to record the membrane potential and clamp current of pyramidal neurons in the prefrontal cortex.Results Compared with group C,the clamp current was significantly increased,and the membrane potential was decreased in group M (P＜0.05).Compared with group M,the clamp current was significantly decreased,and the membrane potential was increased in L + M and R + M groups (P＜0.05),and no significant change was found in the clamp current or membrane potential in group P+M (P＞0.05).Conclusion Melatonin inhibits the excitability of pyramidal neutrons in the prefrontal cortex,and the mechanism is related to activating MT1 R-cAMP-PKA signaling pathway.
