Differentiation types of microglia induced by macrophage colony-stimulating factor: an in vitro cell experiment

VernacularTitle:巨噬细胞集落刺激因子诱导小胶质细胞的分化类型:体外细胞实验
Author: Zhao DAI ¹ ; Haichen CHU ; Lin ZHU ; Yongxin LIANG
Author Information

1. 青岛大学附属医院麻醉科 266000
Keywords: Macrophage colony-stimulating factor; Microglia; Cell differentiation
From: Chinese Journal of Anesthesiology 2019;39(2):206-208
CountryChina
Language:Chinese
Abstract: Objective To study the differentiation types of microglia induced by macrophage colony-stimulating factor (M-CSF).Methods Rat microglia cultured in vitro were inoculated on 6-well plates and divided into 3 groups (n=4 each) using a random number table method when cell confluence reached 70％:blank control group (C group),vehicle control group (P group) and M-CSF group.Group P was incubated with phosphate buffer solution for 7 days and group M-CSF with 20 ng/ml M-CSF for 7 days.The expression of a specific M1 phenotype marker tumor necrosis factor-alpha (TNF-α) and specific M2 phenotype markers interleukin-10 (IL-10) and brain-derived neurotrophic factor (BDNF) was determined by Western blot.Results Compared with C group,the expression of IL-10 and BDNF was significantly upregulated (P＜0.05),and no significant change was found in TNF-α expression in M group (P＞0.05),and no significant change was found in the expression of TNF-α,IL-10 or BDNF in P group (P＞0.05).Conclusion M-CSF can induce microglia to differentiate into a M2 phenotype.