Role of spinal cord TRAF6/NF-κB signaling pathway in development of diabetic neuropathic pain in rats
10.3760/cma.j.issn.0254-1416.2019.02.019
- VernacularTitle:脊髓TRAF6/NF-κB信号通路在大鼠糖尿病神经痛形成中的作用
- Author:
Yunhua WANG
1
;
Wanyou HE
;
Weicheng ZHAO
;
Jieping REN
;
Jian HE
;
Jing WANG
;
Jinhua ZHANG
;
Hanbing WANG
Author Information
1. 佛山市第一人民医院麻醉科 528000
- Keywords:
Spinal cord;
Tumor necrosis factor receptor-associated peptides and proteins;
NF-kappa B;
Diabetes mellitus;
Neuralgia
- From:
Chinese Journal of Anesthesiology
2019;39(2):199-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of spinal cord tumor necrosis factor receptor-associated factor 6 (TRAF6)/nuclear factor kappa B (NF-κB)signaling pathway in the development of diabetic neuropathic pain (DNP)in rats.Methods Clean-grade healthy adult male Sprague-Dawley rats,aged 2 months,weighing 180-230 g,in which IT catheters were implanted,were used in this study.Streptozotocin 60 mg/kg was intraperitoneally injected after IT catheterization to establish the model of DNP.Twelve DNP rats were divided into 2 groups (n =6 each) by a random number table method:DNP group and DNP plus TRAF6 inhibitor group (group DTR).Another 6 age-matched Sprague-Dawley rats were used as normal control group (group NC).The rats in group DC and group DTR received IT injection of dimethyl sulfoxide 10 μl and TRAF6 inhibition 10 μg,respectively,once a day for 7 consecutive days starting from day 21 after establishing the model.The mechanical paw withdrawal threshold (MWT) was determined before establishing the model (T1),on 7,14 and 21 days after establishing the model (T2-4),and on 1,4 and 7 days after IT injection (T5-7).The rats were sacrificed after the last MWT measurement,and the L3-5 segments of the spinal cord were removed for determination of the expression of TRAF6 and NFκB p65 by Western blot.Results Compared with group NC,the MWT at T3-7 in group DC and at T3-6 in group DTR was significantly decreased,and the expression of spinal TRAF6 and NF-κB p65 was up-regulated in DC and DTR groups (P＜0.05).Compared with group DC,the MWT was significantly increased at T6-7,and the expression of spinal TRAF6 and NF-κB p65 was down-regulated in group DTR (P ＜ 0.05).Conclusion Spinal cord TRAF6/NF-κB signaling pathway is involved in the development of DNP in rats.
