Comparative Study in DNA-mediated Vaccination Efficaency Among the Plasmids with Different Promoters.
- Author:
Sun Hwa CHANG
;
Koo Nam YANG
;
Yong Suk JANG
- Publication Type:Comparative Study ; Original Article
- Keywords:
DNA vaccine;
Promoter;
Antibody immune response
- MeSH:
Animals;
Clone Cells;
Creatine Kinase, MM Form;
Gene Expression;
Immunization;
Mice;
Myoblasts;
Ovalbumin;
Plasmids*;
Vaccination*
- From:Korean Journal of Immunology
1998;20(4):375-379
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Plasmid vectors with either RSV or CMV promoter are frequently used for DNA- mediated immunization due to the availability in commercial. Consequently, influence of the vector constituents, such as promoter, enhancer and transcription termination signal etc. on vaccination efficiency is not studied extensively. As an initial attempt to develop an efficient vector system for DNA-rnediated immunization, influence of promoter for antigen gene expression on vaccination efficiency has been analyzed. Initially, plasmids with either B-actin or muscle creatine kinase (MCK) promoter were constructed from the plasmid with prototype CMV promoter. In addition, ovalbumin (OVA) antigen gene has been cloned into each vectors to generate the plasmid vectors with different promoters for induction of the anti-OVA immune responses. Antigen protein expression in antigen gene transfected mouse muscle myoblast cells showed that the level from MCK promoter containing plasmid was slightly higher than those from either CMV or B-actin promoter containing plasmids. Also, the same plasmid turned out to be slightly more efficient than other plasmids in antibody imrnune response induction in vivo, when they were applied both through intramuscularly and intradermally. These results suggest that the commonly used CMV promoter containing plasmid vector could be further modified to develop an efficient vector for DNA-mediated immunization.
