Integration of genome wide association study with gene expression profiling identifies ROR1 as a novel susceptibility gene for coronary artery disease
10.3760/cma.j.issn.0254-9026.2019.07.004
- VernacularTitle:转录组学结合全基因组关联分析鉴定ROR1为冠心病新易感基因
- Author:
Yang LI
1
;
Han YAN
;
Xiaoli TIAN
Author Information
1. 首都医科大学附属北京安贞医院北京市心肺血管疾病研究所心血管生物研究室
- Keywords:
Genomics;
Coronary disease;
Genetic predisposition to disease
- From:
Chinese Journal of Geriatrics
2019;38(7):727-732
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify new susceptibility genes for coronary artery disease(CAD)by integrating genome wide association study and gene expression profiling.Methods On account of our data of genome wide association study(GWAS)on CAD,our integrative analysis of gene expression profiles and GWAS for CAD was conducted,and we obtained candidate genes and single nucleotide polymorphisms(SNPs) for a multicenter case-control genetic association study.Using a DNA ligase chain reaction-based genotyping method,we validated these candidate loci in two independent populations.Results Firstly,we selected 21 SNPs for 21 candidate genes.Using a DNA ligase chain reaction-based genotyping method,we validated these candidate loci in two independent populations,including population 1(495 cases and 492 control)and population 2(810 cases and 853 control).And we identified an intragenic SNP rs11208367 (ROR 1),which was significantly associated with CAD.Meta confluence analysis in combination of GWAS and two validation populations showed that the P value of rs11208367 was 2.00 × 10-4,which was still significant after Bonferroni multiple test correction (0.05/21 =2.38 × 10-3).Conclusions The rs11208367 SNP of ROR1gene is significantly associated with CAD,indicating that ROR1is a novel susceptibility gene for CAD.
