Antigenic diversity of Theileria major piroplasm surface protein gene in Jeju black cattle.
- Author:
Myung Soon KO
1
;
Kyoung Kap LEE
;
Kyu Kye HWANG
;
Byung Sun KIM
;
Gui Cheol CHOI
;
Young Min YUN
Author Information
- Publication Type:Original Article ; Comparative Study
- Keywords: cattle; MPSP gene; piroplasma; surface protein; theileriosis
- MeSH: Animals; Antigens, Protozoan/*genetics; Base Sequence; Cattle; DNA Primers/genetics; *Genetic Variation; Korea; Molecular Sequence Data; *Phylogeny; Protozoan Proteins/*genetics; Sequence Analysis, DNA; Theileria/*genetics; Theileriasis/*parasitology
- From:Journal of Veterinary Science 2008;9(2):155-160
- CountryRepublic of Korea
- Language:English
- Abstract: Piroplasms are tick-transmitted, intracellular, hemoprotozoan parasites that cause anorexia, fever, anemia, and icterus. Theileriosis is caused by Theileria sergenti and causes major economic losses in grazing cattle in Japan and Korea. In May 2003, we examined the antigenic diversity of the major piroplasm surface protein (MPSP) gene in 35 healthy Jeju black cattle that were born and raised at the National Institute of Subtropical Agriculture. On microscopic examination of Giemsa-stained blood smears, 9 of 35 cattle had intra-erythrocytic piroplasms. Hematological data were within normal range for all 35 cattle. Amplification of DNA from all blood samples using universal MPSP gene primers showed mixed infections with C, I, and B type Theileria spp. Type C was identified in 20 of 35 blood samples, and type B was identified in 17 samples. Allelic variation was seen in type B.
