Effect of microRNA-1290 on invasion and metastasis of pancreatic cancer
10.3760/cma.j.issn.1007-8118.2019.06.014
- VernacularTitle:微RNA-1290对胰腺癌侵袭转移的影响
- Author:
Zili CHEN
1
;
Yifei MA
;
Yaozhen PAN
;
Chao YU
;
Huajian GU
;
Changhao ZHU
;
Chengyi SUN
Author Information
1. 贵州医科大学附属医院肝胆外科 贵州医科大学肝胆胰脾重点实验室 贵州贵阳550004
- Keywords:
MicroRNA;
Pancreatic neoplasm;
Neoplasm metastasis;
MicroRNA-1290;
Matrix metalloproteinase 2 (MMP-2);
Cyclooxygenase 2 (COX-2)
- From:
Chinese Journal of Hepatobiliary Surgery
2019;25(6):457-461
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of microRNA-1290 in pancreatic cancer and its role in invasion and metastasis of pancreatic cancer.Methods The expression of microRNA-1290 in pancreatic cancer tissue microarray and pancreatic cancer cell lines (AsPC-1,BxPC-3,Capan-2,Panc-1,and MIA PaCa-2) were detected by immunohistochemistry and QT-PCR.The pancreatic cancer cell lines Panc-1 and MIA PaCa-2 in logarithmic growth phase were treated with microRNA-1290 inhibitor,and the invasion and metastasis ability of pancreatic cancer cells were detected by Transwell and wound healing asssay.Western Blot was used to detect the expression of invasion and metastasis-associated proteins cyclooxygenase 2 (COX-2) and matrix metalloproteinase 2(MMP-2) in pancreatic cancer cell lines.Results (1) The expression of microRNA-1290 in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues and adjacent tissues (P ＜ 0.05).(2) Compared with pancreatic normal epithelial cells (HPDE),the expression of microRNA-1290 was significantly higher in different pancreatic cancer cell lines (P ＜ 0.05).The expression level of MicroRNA-1290 in Panc-1 and MIAPaCa-2 pancreatic cancer cells was significantly higher than that in other pancreatic cancer cell lines (P ＜ 0.05).(3) The number of invasive and metastatic cells was significantly decreased after treatment with microRNA-1290 inhibitor (P ＜0.05).(4) The expression of MMP-2 and COX-2 were decreased in Panc-1 and MIAPaCa-2 pancreatic cancer cells treated with MicroRNA-1290 inhibitor.Conclusion The expression of MMP-2 and COX-2 may be involved in the invasion and metastasis of pancreatic cancer cell by regulating the expression of microRNA-1290 in pancreatic cancer.
