Silencing LncRNA HOXD-AS1 enhances the radiosensitivity of H460 cells by regulating miR-454-3p expression
10.3760/cma.j.issn.1004-4221.2019.08.012
- VernacularTitle:沉默LncRNA HOXD-AS1调控miR-454-3p表达增加H460细胞放射敏感性
- Author:
Qiufeng PENG
1
;
Kui YE
;
Jingzhen GAO
;
Aimin XING
Author Information
1. 天津市第四中心医院呼吸二科300140
- Keywords:
LncRNA HOXD-AS1 gene;
miR-454-3p gene;
Lung cancer cell line;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2019;28(8):610-611
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of LncRNA HOXD-AS1 on the radiosensitivity of H460 cells by targeting the regulation of miR-454-3p expression. Methods H460 cells were irradiated with 0, 2, 4, 6 and 8 Gy, and the expression levels of HOXD-AS1 and miR-454-3p were detected by qRT-PCR. Cell apoptosis rate were measured by flow cytometry experiments. Cell cloning experiments were used to detect cell radiosensitivity. The protein expression levels of Caspase-3, Cyclin D1 and γ-H2AX were detected by Western blot. Results The expression of HOXD-AS1 in H460 cells was significantly increased after X-ray irradiation ( P<0.05) , while the expression of miR-454-3p was significantly decreased ( P<0.05) . Silencing HOXD-AS1 significantly promoted apoptosis, increased radiosensitivity of lung cancer cells, promoted Caspase-3, γ-H2AX protein expression, and inhibited Cyclin D1 expression. HOXD-AS1 could target the expression of miR-454-3p. Conclusion Silencing HOXD-AS1 can promote the apoptosis of lung cancer cells and inhibit cell survival by targeting miR-454-3p to increase the radiosensitivity of lung cancer cells.
