Dose-response relationship of radiation-induced pulmonary fibrosis in mouse models based on CT-derived parameters
10.3760/cma.j.issn.1004-4221.2019.08.010
- VernacularTitle:基于CT参数分析模型评估辐射诱导小鼠肺纤维化剂量-效应关系研究
- Author:
Meijuan ZHOU
1
;
Zhaoming ZHOU
;
Lei WEN
;
Hao LIU
;
Liji CAO
;
Shun LU
;
Ziyao LI
;
Zhouguang HUI
;
Linbo CAI
;
Ming CHEN
;
Longhua CHEN
;
Cheng ZHOU
Author Information
1. 南方医科大学公共卫生学院放射医学系
- Keywords:
Radiation-induced lung fibrosis;
Radiological assessment;
Radiobiology;
Dose-response effect;
Mouse
- From:
Chinese Journal of Radiation Oncology
2019;28(8):601-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the radiation induced pulmonary fibrosis with a dose-response mouse model, based on the CT image changes of pulmonary fibrosis.Methods Female C57BL6 mice aged 8-10 weeks were randomly divided into 20 Gy or escalated doses of X-ray whole thoracic irradiation ( WTI) groups. CT scan was performed at different time points before and after radiation. The average lung density and lung volume changes were obtained by three-dimensional segmentation algorithm. After gene chip and pathological validation, the parameters of CT scan were subject to the establishment of logistic regression model. Results At the endpoint of 24 weeks post-irradiation, the lung density in the 20 Gy irradiation group was (-289.81± 12.06) HU, significantly increased compared with (-377.97± 6.24) HU in the control group ( P<0.001) . The lung volume was ( 0.66±0.01) cm3 in the control group, significantly larger than ( 0.44±0.03) cm3 in the irradiated mice ( P<0.001) . The results of quantitative imaging analysis were in accordance with the findings of HE and Mason staining, which were positively correlated with the fibrosis-related biomarkers at the transcriptional level ( all R2=0.75, all P<0.001) . The ED50 for increased lung density was found to be ( 13.64± 0.14) Gy ( R2=0.99, P<0.001) and ( 16.17± 4.36) Gy ( R2=0.89, P<0.001) for decreased lung volume according to the logistic regression model. Conclusions Quantitative CT measurement of lung density and volume are reliable imaging parameters to evaluate the degree of radiation-induced pulmonary fibrosis in mouse models. The dose-response mouse models with pulmonary fibrosis changes can provide experimental basis for comparative analysis of high-dose hypofractioned irradiation-and half-lung irradiation-induced pulmonary fibrosis.
