The role of ceramide in radiation-induced bystander effect on germ cells in Caenorhabditis elegans
10.3760/cma.j.issn.0254-5098.2019.06.002
- VernacularTitle:神经酰胺在辐射诱导的秀丽隐杆线虫生殖细胞旁效应中的机制研究
- Author:
Guangmin XU
1
,
2
;
Yaning YANG
;
Shengmin XU
;
Lijun WU
Author Information
1. 中国科学院合肥物质科学研究院强磁场与离子束物理生物学重点实验室 230031
2. 中国科学技术大学
- Keywords:
Radiation induced bystander effect (RIBE);
Ceramide;
Caenorhabditis elegans;
Germ cell;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2019;39(6):408-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of ceramide in radiation induced bystander effect (RIBE) in vivo by irradiating Caenorhabditis elegans with proton microbeam.Methods The posterior pharynx of wide-type N2 and genetically mutated L4-staged worms was irradiated with 2 000 particles,then germ cell apoptosis and gene expression were analyzed.Results Point-fixed radiation to posterior pharynx of N2 worms significantly increased the number of apoptotic germ cells (t =9.007,P＜0.05),but not in the worms with deletion mutants of ceramide synthase (CerS) genes (hyl-1 and lagr-1) (P＞0.05).Realtime quantitative PCR assay indicated that in both N2 and lagr-1 (gk327);hyl-1 (ok976) worms,the genes of egl-1 and ced-13 in core apoptosis pathway were up-regulated after radiation to posterior pharynx,without statistical significance between these two strains (P＞0.05).The gene expression levels of hyl-1 and lagr-1 were increased in both N2 and hus-1 (op241) worms after radiation with no statistical significant difference between two strains (P＞0.05).Furthermore,radiation-induced germ cell apoptosis was increased in abl-1 (ok171) worms (t=13.241,P＜0.05),rather than in lagr-1(gk327);hyl-1(ok976);abl-1(ok171) worms (t=13.462,P＜0.05).Conclusions Ceramide is required for RIBE on germ cell apoptosis in C.elegans,and it might play function together with egl-1 and ced-13 in core apoptosis pathway and HUS-1 in DNA damage response pathway.But ceramide had antagonistic relationship with anti-apoptotic protein abl-1 in germ cell apoptosis.
