Effect of low-frequency pulsed magnetic field on blood-brain barrier permeability of rat hippocampus
10.3760/cma.j.issn.0254-5098.2019.06.001
- VernacularTitle:低频脉冲磁场对大鼠海马血脑屏障通透性的影响
- Author:
Qin CHEN
1
;
Peng GAO
;
Junfeng HU
;
Guirong DING
;
Guozhen GUO
Author Information
1. 空军军医大学军事预防医学系辐射防护医学教研室
- Keywords:
Pulsed magnetic field;
Rats;
Hippocampus;
Blood brain barrier;
Tight junction protein
- From:
Chinese Journal of Radiological Medicine and Protection
2019;39(6):401-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pulsed magnetic radiation on BBB permeability of hippocampus in Sprague-Dawley rats at different exposure intensity.Methods A total of 72 male SD rats were randomly divided into sham exposure group,positive control group and magnetic field treatment groups (100,400,800,1 200 mT,30 pulses each group).At three hours after exposure,the morphological structure of hippocampus was evaluated by HE staining,the extravasation of albumin around microvessels in rat hippocampus was detected immunohistochemically,the EB extravasation around microvessels was observed with Evans blue (EB) fluorescence method,and the levels of BBB-related protein ZO-1 and Occludin in hippocampus were measured with Western blot assay.Results Compared with the control group,no significant change in the hippocampus morphology structure,the extravasation of albumin and EB around the microvessels were observed after the pulsed magnetic exposures.The protein levels of ZO-1 and Occludin had no changes in the exposed groups (P＞ 0.05) except that ZO-1 was significantly reduced in 1 200 mT exposure group (t =14.26,P ＜ 0.05).Conclusions Low-frequency pulsed magnetic field could not affect the permeability of BBB in SD rats but impairs the integrity of BBB at 1 200 mT.
