Effects of parthenolide on estradiol?synthesizing enzyme, ER isoforms and VEGF in human endometriotic stromal cells
10.3760/cma.j.issn.0529?567x.2019.07.006
- VernacularTitle:小白菊内酯对异位子宫内膜间质细胞雌激素合成相关酶、ER亚型、VEGF表达的影响
- Author:
Han SONG
1
;
Yan HUANG
;
Chao PENG
;
Qing XUE
;
Yingfang ZHOU
Author Information
1. 北京大学第一医院妇产科100034
- Keywords:
Endometriosis;
Sesquiterpenes;
Stromal cells;
Estrogen receptor;
Aromatase;
Parthenolide
- From:
Chinese Journal of Obstetrics and Gynecology
2019;54(7):464-469
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of parthenolide on estradiol?synthesizing enzyme, steroidogenic acute regulatory protein (StAR), and ER isoforms,VEGF in human endometriotic stromal cells. Methods Primary endometriotic stromal cells were treated with different concentrations (1, 5, 10 and 20 μmol/L) of parthenolide. The mRNA of StAR, ER isoforms (ERα and ERβ), PR, vascular endothelial growth factor (VEGF), interleukin?6 (IL?6), tumour necrosis factor?α (TNFα), tumour necrosis factor receptor (TNFR) 1, TNFR2 were measured by real?time PCR. The levels of estradiol and progesterone in the cell supernatant were measured by ELISA. Results Different concentrations of parthenolide could up?regulate the mRNA of StAR in primary endometriotic stromal cells (F=5.722, P<0.05); the mRNA of StAR in the group of 20 μmol/L was significantly higher than that of the control group [2.6 ± 0.3 versus 1.0, P<0.01]. Different concentrations of parthenolide could down?regulate the mRNA of ERα (F=6.921, P<0.01); the mRNA of ERα in the group of 20 μmol/L and 10 μmol/L were significantly lower than those of the control group [0.2 ± 0.3 versus 0.3 ± 0.3 versus 1.0, all P<0.05]. Different concentrations of parthenolide could down?regulate the ratios of ERα/ERβ mRNA levels (F=4.209, P<0.05). Different concentrations of parthenolide could up?regulate the mRNA of VEGF and TNFR1 (F=10.964, P<0.01; F=7.286, P<0.01). There were no statiscal significances with different concentrations of parthenolide on the mRNA of ERβ, PR, IL?6, TNFα and TNFR2, and the levels of estradiol and progesterone in the cell supernatant (all P>0.05). Conclusions Parthenolide may regulate the expression of estradiol?synthesizing enzyme, ER isoforms and angiogenesis in endometriotic stromal cells. Parthenolide may promote the development of endometriosis.
