Relationship between cervical lesions and the type?specific viral load of high risk HPV reflected by the Ct value of Cobas 4800 HPV system
10.3760/cma.j.issn.0529-567x.2019.07.005
- VernacularTitle:Cobas 4800 HPV检测的Ct值反映的型别特异性HR?HPV亚型病毒载量与子宫颈病变关系的研究
- Author:
Lüfang DUAN
1
;
Hui DU
;
Aimin XIAO
;
Chun WANG
;
Xia HUANG
;
Meifang ZHAO
;
Hongjian MEN
;
Ruifang WU
Author Information
1. 北京大学深圳医院妇产科暨深圳市女性重大疾病早期诊断技术重点实验室518036
- Keywords:
Human papillomavirus 16;
Viral load;
Uterine cervical neoplasms;
Precancerous conditions;
Prognosis
- From:
Chinese Journal of Obstetrics and Gynecology
2019;54(7):458-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between cervical lesions and high risk HPV (HR-HPV) viral load reflected by the cycle threshold (Ct) values of Cobas 4800 HPV (Cobas 4800) system. Methods From August 2016 to September 2017, 7 000 women from Shenzhen, were recruited for cervical cancer screening with Cobas 4800 system and cytology co-test. Colposcope biopsies were performed on women who were positive of HPV 16, 18, and positive of HPV types other than 16, 18 with cytology [≥atypical squamous cell of undetermined signification (ASCUS)], or HPV negative but abnormal of cytology [≥low grade squamous intraepithelial lesion (LSIL)]. The Ct values of HPV 16, 18 and all combined other types coming from Cobas 4800 system were used as an indicator of viral load to analyze the relationship between type-specific HPV load and the cervical lesions. Results (1) Among the 7 000 screening women, 370 cases were positive for cervical cancer screening, 325 of them underwent colposcope biopsies, and coloposcopy referred rate was 87.8% (325/370). Among 325 women undergoing cervical biopsy, pathological diagnosis was 119 cases of normal cervical cervix, 151 cases of LSIL, and 55 cases of high-grade squamous intraepithelial lesion (HSIL) and above (HSIL+; including 53 cases of HSIL, 1 case of cervical adenocarcinoma, and 1 case of cervical squamous cell carcinoma). (2) The Ct value of HPV 16 was inversely correlated with the upgrading of the lesions (r=-0.617, P=0.000), and significant different among normal cervix,LSIL and HSIL+(35.4±4.5 vs 31.0±6.0 vs 26.5±4.0; F=25.537, P=0.000). There was no correlation between Ct value of HPV 18 and cervical lesions (r=-0.021, P=0.902). The Ct value of other 12 HPV types was statistically difference among normal normal cervix , HSIL+and cervicitis (33.0±5.3 vs 29.9±7.2 vs 29.8±5.8; F=5.087, P=0.007). Among them, LSIL and HSIL+ were significantly lower than normal cervix (P<0.05), but there was no significant difference between LSIL and HSIL+(P>0.05). Conclusion The Ct value of HPV 16 detecting in Cobas 4800 system as an indicator of virus load obviously correlates with different grades of cervical lesions, therefore could be a reference of cervical lesion existence and an indicator of lesion prognosis.
