Effects of Sinomenine on Expressions of TLR-2, TLR-4 and MyD88 in Cartilage of Rabbit Knee Osteoarthritis Model
10.3969/j.issn.1005-5304.2018.09.012
- VernacularTitle:青藤碱对兔膝骨关节炎模型软骨Toll样受体2、4及髓样分化因子88表达的影响
- Author:
Jie ZHENG
1
;
Li-Ping ZHAO
;
Ya-Li HU
;
Pu-Wei YUAN
Author Information
1. 陕西中医药大学
- Keywords:
sinomenine;
osteoarthritis;
innate immune;
toll-like receptor;
myeloid differentiation factor 88;
rabbits
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(9):49-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different doses of sinomenine on toll-like receptor (TLR) 2, TLR4 and myeloid differentiation factor 88 (Myd88) in cartilage of rabbit knee osteoarthritis model; To discuss its mechanism of action. Methods Knee osteoarthritis model was established by Hulth method. Experimental rabbits were randomly divided into control group, model group, sinomenine low-, medium-and high-dose groups. Blank group received no processing. Model group, sinomenine low-, medium-and high-dose groups received intra-articular injection of normal saline and sinomenine 0.2 mL (5 mg), 0.35 mL (8.75 mg) and 0.5 mL (12.5 mg) respectively, for 10 times. Real-time fluorescence quantitative PCR and Western blot were used to detect m RNA and protein expressions of TLR2, TLR4 and My D88 in cartilage. Results m RNA and protein expressions of TLR2, TLR4 and MyD88 in model group significantly increased compared with control group (P＜0.01); compared with model group, m RNA and protein expressions of TLR2, TLR4 and My D88 in sinomenine medium-and high-dose groups decreased significantly (P＜0.05, P＜0.01). Conclusion Sinomenine can inhibit the cartilage immune response of rabbit knee osteoarthritis by down-regulating expressions of key molecules in the TLR/My D88 pathway.
