The effect of entinostat on NK killing effect on non-small cell lung cancer in vitro
10.3969/j.issn.1000-8179.2019.08.348
- VernacularTitle:Entinostat影响NK细胞对非小细胞肺癌杀伤作用的体外研究*
- Author:
Yinghui SUN
1
;
Xiaodong XIE
;
Mingyue OUYANG
;
Shuang SONG
;
Ji YANG
;
Huiying YU
Author Information
1. 北部战区总医院医学实验科(沈阳市110016)
- Keywords:
entinostat;
non-small cell lung cancer (NSCLC);
NKG2D ligands;
NK cell
- From:
Chinese Journal of Clinical Oncology
2019;46(8):378-383
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the Effects of entinostat on the expression of NKG2D ligands in the non-small cell lung cancer (NSCLC) cell lines, A549 and HCC-827, and to detect the effect of entinostat-mediated NK cell killing of A549 and HCC-827 cells. Meth-ods: The effect of entinostat on A549 and HCC-827 cell proliferation was measured by MTT assay. Flow cytometry was used to detect the expression of NKG2D ligands. mRNA levels of the ligands were detected by RT-PCR . The level of soluble MICA in cell culture super-natant was evaluated by ELISA. The cytotoxicity of NK cells against A549 and HCC-827 cell lines (treated with entinostat) was assessed using lactate dehydrogenase release assay. Results: Entinostat showed a time-and dose-dependent inhibition effect on the prolifera-tion of A549 and HCC-827 cell lines. The expression of NKG2D ligands and mRNA transcription levels of MICA and MICB were en-hanced after treatment with 0.5, 1μmol/L entinostat for 48 h. The soluble MICA level in A549 cell culture supernatant was increased by 1μmol/L entinostat. The sensitivity of HCC-827 cells to NK cells was enhanced upon treatment with 0.5, 1μmol/L entinostat. Con-clusions: entinostat enhanced the killing effect of NK cells on non-small cell lung cancer cells by up-regulating the expression of NKG2D ligands. This provides a new method and theory for the treatment of NSCLC.
