The expression of microRNA-145 in cervical cancer and its inhibitory effects on Wnt/β-catenin signaling pathway
10.3969/j.issn.1000-8179.2019.03.197
- VernacularTitle:microRNA-145在宫颈癌中的表达及其对Wnt/β-catenin信号通路的抑制作用
- Author:
Xiangqing WANG
1
;
Zhenjun MA
;
Hongyun BAO
;
Fangfang KONG
Author Information
1. 解放军464医院妇产科(天津市300381
- Keywords:
microRNA-145;
cervical cancer;
Wnt/β-catenin signaling pathway;
β-catenin;
TCF/LEF;
Cateninδ-1
- From:
Chinese Journal of Clinical Oncology
2019;46(3):117-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the clinic-pathological significance of microRNA-145 expression in human cervical cancer and its ef-fects on Wnt/β-catenin signaling pathway. Methods: Real-time PCR was used to detect the expression of microRNA-145 in 62 cervical cancer samples. The correlation between microRNA-145 expression and the clinic-pathological parameters and its prognostic signifi-cance was analyzed. MicroRNA-145-expressing plasmid and non-sense plasmid were transfected into human cervical cancer HeLa cells, assigned into overexpressed microRNA-145 group and control group. Immunofluorescence staining was performed to detect the expression location of β-catenin. Top Flash luciferase reporter assay was performed to investigate the effects of microRNA-145 on the transcriptional activity of TCF/LEF and direct interactions with Cateninδ-1. Western blot was used to detect the effects of microRNA-145 on the expression of Cateninδ-1, C-MYC, and CyclinD1. Results: The patients with low microRNA-145 expression showed poorer prognosis [(41.28 ± 2.00) months vs . (46.06 ± 0.95) months, P<0.05]. β-catenin immunofluorescence was distributed within the cyto-plasm in the microRNA-145-overexpressed HeLa cells, but mainly within the nucleus and cytoplasm in the control cells. The luciferase reporter system indicated that the transcriptional activity of TCF/LEF was inhibited in the microRNA-145-overexpressed HeLa cells, and validated Cateninδ-1 was a target of miR-145. The expression of Cateninδ-1, C-MYC, and CyclinD1 was decreased in the microRNA-145-overexpressed HeLa cells. Conclusions: microRNA-145 may inhibit cervical cancer progression via Cateninδ-1 and inhibit the Wnt/β-catenin signaling pathway.
