Effects of astragaloside Ⅳ on activity and proliferative function of endothelial progenitor cells
10.3760/cma.j.issn.1008-1372.2019.08.010
- VernacularTitle:黄芪甲苷对人内皮祖细胞活性及增殖功能的影响
- Author:
Wu XIONG
1
;
Sihong CHEN
;
Yumeng WANG
;
Shuangxi YANG
;
Yicheng YU
;
Yi HUANG
;
Tingting WANG
;
Honglian ZHANG
Author Information
1. 湖南中医药大学第一附属医院烧伤疮疡整形外科
- Keywords:
Astragalus membranaceus;
Saponins;
Endothelial progenitor cells;
Cell proliferation;
Cell survival;
In vitro
- From:
Journal of Chinese Physician
2019;21(8):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective is to probe into the effects of astragaloside Ⅳ (AS-Ⅳ) on activity and proliferative function of endothelial progenitor cells (EPCs),which lays a foundation for further study on the effects of AS-Ⅳ on vascular neovascularization mediated by endothelial progenitor cells.Methods The mononuclear cells were isolated by the density gradient centrifugation in umbilical cord blood of full-term healthy infants,and EPCs were obtained by subculture and cell identification when the cells presented spindle shapes.The obtained EPCs were randomly divided into the experimental group and the control group.In the experimental group,EPCs were cultured by AS-Ⅳ with different concentration gradients (25 mg/L,50 mg/L,100 mg/L,200 mg/L and 400 mg/L),while in the control group,they were treated with the same amount of phosphate buffer saline (PBS) solutions.The effects of AS-Ⅳ on the proliferation of endothelial progenitor cells was studied by cell counting kit-8 (CCK-8) cell proliferation experiment,and the activity rate of EPCs cells was measured at the optimum concentration of EPCs proliferation.Results EPCs were successfully obtained after confirming nuclear staining test of CD31 antibody and 4',6-diamidi-no-2-phenylindole (DAPI).Further study showed that AS-Ⅳ can promote the proliferation of EPCs,and its optimal concentration of EPCs proliferation is 100 mg/L.Compared with the normal control group,the activity rate of endothelial progenitor cells after intervention of AS-Ⅳ was 98.7％,higher than 98.12％ in the control group,with significant difference (x2 =49.59,P ＜0.01).Conclusions AS-Ⅳ can enhance the activity of human EPCs and promote their proliferation in vitro.
