Association Study between Schizophrenia and Monocyte Chemoattractant Protein-1(MCP-1) Gene Polymorphism.
- Author:
Kyoo In CHUNG
1
;
Chi Un PAE
;
Ja Hyeun JO
;
Jung Jin KIM
;
Chang Uk LEE
;
Soo Jung LEE
;
Chul LEE
;
In Ho PAIK
;
Seung Kyu BANG
Author Information
1. Department of Psychiatry, St. Paul's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Schizophrenia;
Monocyte chemoattractant protein-1 gene
- MeSH:
Age of Onset;
Alleles;
Chemokine CCL2;
Diagnostic and Statistical Manual of Mental Disorders;
DNA;
Endopeptidase K;
Genotype;
Humans;
Monocytes*;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Promoter Regions, Genetic;
Schizophrenia*
- From:Journal of Korean Neuropsychiatric Association
2003;42(4):454-460
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: This study was aimed at comparing the Monocyte Chemoattractant Protein-1 (MCP-1) gene polymorphic variants of schizophrenics with those of normal controls, and to investigate its contribution to the development of schizophrenia. METHODS: One hundred and thirty five schizophrenics in accordance with DSM-IV criteria and one hundred and fourteen healthy individuals participated in this study. DNA was extracted from peripheral blood using proteinase K, and the MCP-1 gene promoter region was amplified by polymerase chain reaction (PCR). Gene typing was analyzed by restriction fragment length polymorphism (RFLP) using restriction enzyme PvuII. RESULTS: Distribution of the genotypes and alleles of MCP-1 gene in the patient group was not significantly different from that of the control group (p=0.145 and p=0.122, respectively). There was difference in the frequencies of genotypes between positive and negative subgroup (p=0.035), but no difference in frequencies of alleles between the two subgroups (p=0.078). There was no difference in scores of PANSS general, positive and negative subscale, the age of onset, the presence or absence of family history between patients with or without -2518G allele (p=0.979, p=0.378, p=0.056, p=0.256 and p=0.301, respectively). CONCLUSION: We suggest that the polymorphism in the MCP-1 promotor gene may be the possible marker for subgouping of negative type schizophrenia.
