Comparative analysis of lncRNA-mRNA co-expression between Keshan disease and dilated cardiomyopathy
10.3760/cma.j.issn.2095-4255.2019.05.005
- VernacularTitle:克山病与扩张型心肌病的lncRNA-mRNA共表达对比研究
- Author:
Guangyong HUANG
1
;
Youzhang XIANG
;
Jingwen LIU
;
Yuehai WANG
;
Jing WANG
;
Miaomiao CAO
;
Xuesong WANG
;
Guanfeng CHONG
;
Wenbo YANG
Author Information
1. 山东大学附属聊城市人民医院心内科 252000
- Keywords:
Keshan disease;
Dilated cardiomyopathy;
RNA-Seq
- From:
Chinese Journal of Endemiology
2019;38(5):361-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective By constructing the differential expression profile of lncRNA/mRNA in peripheral blood plasma of patients with Keshan disease (KSD) and dilated cardiomyopathy (DCM),to explore the commonality and characteristics of the two diseases in molecular mechanism.Methods Ten patients with chronic KSD were selected in the severe disease area of KSD in Shandong Province,and 10 cases of DCM and 10 healthy subjects (control group) were selected in non-KSD area.Blood of elbow vein was collected and plasma was separated.RNA-seq technology was used to construct the differential lncRNA/mRNA expression profile between KSD and control group,DCM and control group,and co-expression and specific expression of partial genes in KSD and DCM were analyzed through Wien analysis.The lncRNA-mRNA co-expression network maps of specific part of KSD,specific part of DCM and common part of the two diseases were constructed,and Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were applied to distinguish the biological function of the two diseases.Results Compared with control group,102 dysregulated mRNAs and 22 dysregulated lncRNAs showed the same trend in KSD and DCM.And 3 606 mRNAs and 451 lncRNAs were only differentially expressed in KSD group,217 mRNAs and 137 lncRNAs were only differentially expressed in DCM group.The differentially expressed lncRNA/mRNA shared between the KSD and DCM groups were mainly about viral transcription,immuno-inflammatory response,oxidative stress signaling pathways.The KSD specific lncRNA/mRNA mainly participated in cell membrane damage and viral myocarditis.The DCM specific lncRNA/mRNA mainly regulated mitochondrial structure and oxidative phosphorylation related enzymes.Conclusion The differentially expressed lncRNA/mRNA shared in KSD and DCM groups are mainly involved in viral transcription,oxidative stress signaling pathways;KSD specific lncRNA/mRNA are mainly related to cell membrane damage and viral myocarditis;DCM specific lncRNA/mRNA mainly regulate mitochondrial structure.
