Expression level and clinical significance of MicroRNA-15b in premenopausal endometrial carcinoma
10.3760/cma.j.issn.1673-4904.2019.08.007
- VernacularTitle:绝经前子宫内膜癌组织微小RNA-15b的表达水平及临床意义
- Author:
Jing NIU
1
;
Jing ZHANG
;
Cuipei HAO
;
Xiaole DAI
;
Lin CHEN
Author Information
1. 陕西省人民医院妇科
- Keywords:
Endometrial Neoplasms;
Premenopausal;
MicroRNA-15b
- From:
Chinese Journal of Postgraduates of Medicine
2019;42(8):693-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression level of MicroRNA-15b in premenopausal endometrial carcinoma and analyze its relationship with pathological parameters. Methods From March 2016 to June 2018, 64 cases of premenopausal endometrial carcinoma tissues and 28 cases of premenopausal normal endometrial tissues that were surgically resected and examined in Shaanxi People′s Hospital were collected. The expression of microrna- 15b was detected by reverse transcription polymerase chain reaction (rt-pcr), and the relationship between the expression level of microrna-15b and the pathological parameters of premenopausal endometrial carcinoma was analyzed. Results The relative expression level of microrna-15b in premenopausal endometrial cancer (1.38 ± 0.28) was lower than that in normal endometrial tissue (2.55 ± 0.36), and the difference was statistically significant (P<0.05). MicroRNA-15b expression level in premenopausal endometrial carcinoma tissue of Ⅲ period, infiltration depth 1/2 or more muscle layer, lymph node metastasis, ER negative and negative PR was significantly lower than that of stage Ⅰ ～ Ⅱ, < 1/2 muscularis infiltration depth, no lymph node metastasis, and positive ER and PR(P < 0.05). However, there was no significant difference in the expression level of microrna-15b in premenopausal endometrial cancer tissues with different ages, pathological types and pathological grades (P>0.05). Conclusions The expression of MicroRNA-15b in premenopausal endometrial carcinoma is low, and it is closely related to pathological stage, depth of invasion, lymph node metastasis and hormone receptor. Up-regulation of microRNA-15b expression may become a new treatment direction for premenopausal endometrial cancer.
