A study on the association between vascular endothelial growth factor A polymorphisms and necrotizing enterocolitis
10.3760/cma.j.issn.2096-2932.2019.04.005
- VernacularTitle:血管内皮生长因子A基因多态性与新生儿坏死性小肠结肠炎相关性研究
- Author:
Xiaoyan GAO
1
;
Yiheng DAI
;
Weidong LIU
;
Sitao LI
;
Xin XIAO
Author Information
1. 佛山市妇幼保健院新生儿科 528000
- Keywords:
Vascular endothelial growth factor A;
Eenterocolitis,necrotizing;
Genotype;
Polymorphism,single nucleotide;
Infant,premature
- From:Chinese Journal of Neonatology
2019;34(4):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor A (VEGFA) gene and neonatal necrotizing enterocolitis (NEC).Method From August 2014 to December 2016,preterm infants with a ≥ Ⅱ stage (Modified Bell staging criteria) of NEC admitted to our hospitals were assigned as NEC group.Preterm infants without NEC with similar gestational age and body weight during the same period were assigned as the control group.SNPs of VEGFA including rs1005230,rs833067,rs3025010,rs3025035,rs3025036,rs10434,and rs6905288 were analyzed using SEQUENOM MassARRAY platform and multiplex allele-specific PCR.The concentration of VEGFA in the plasma of the two groups was examined using enzyme-linked immunosorbent assay (ELISA).Result A total of 110 infants were reviewed,including 30 infants in NEC group and 80 infants in the control group.The results showed a significant association of the minor allele frequency (MAF) for T in rs1005230 and C in rs833067 with NEC.The frequencies of C/T (OR=4.810,95％CI 1.742～13.278) and C/T-T/T (OR=4.892,95％CI 1.801～13.246) genotypes in rs1005230,and frequencies of T/C (OR=4.373,95％CI 1.578～12.129) and T/C-C/C (OR=4.000,95％CI 1.484～10.828) genotypes in rs833067 were significantly higher in NEC group than the control group (P＜0.05).Infants with MAF in rs1005230 and rs833067 had significantly lower plasma level of VEGFA than infants without MAF (P＜0.01).Conclusion The SNPs of rs1005230 and rs833067 may be associated with lower level of VEGFA in plasma and higher risk for NEC.
