Effects of different concentration oxygen on the lung development of neonatal rats
10.3760/cma.j.issn.1673-4912.2019.08.012
- VernacularTitle:不同浓度氧气对新生大鼠肺发育的影响
- Author:
Yanli SUN
1
;
Wanjie HUANG
;
Xinyi ZHAO
;
Xin TONG
Author Information
1. 中国医科大学附属第四医院儿科
- Keywords:
Oxygen inhalation therapy;
Bronchopulmonary dysplasia;
Lung development;
Rat
- From:
Chinese Pediatric Emergency Medicine
2019;26(8):608-613
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentration hyperoxic exposure on the lung development of neonatal rats for providing theoretical basis for the preparation of bronchopulmonary dys-plasia model. Methods A total of 128 newborn rats were randomly and equally assigned to one of the fol-lowing four groups:group A(FiO2 >0. 9,n＝32),group B ( FiO2 ＝0. 6,n＝32),group C(FiO2 ＝0. 4,n＝32) and the air group(21% O2,n＝32). Lung tissue were collected at day 3,7,14 and 21 for histological analysis. Body weights were recorded,pulmonary morphology and radical alveoli count(RAC),mean alveolar diameter(MAD),alveolar septal thickness(AST) were carried out. Results Compared with the air group of the same time,the body weight of group A significantly decreased at 3 d( P <0. 05),the body weight of group B significantly decreased at 7 d(P<0. 05),the body weight of group C slightly decreased at every time point,but there was no statistical significance(P>0. 05). HE staining showed that the alveolar cavity signifi-cantly increased,alveolar structure was simplified and the alveolar spacing was thickened in different degrees in group A and group B. Furthermore,the reduction in the number of alveoli was more obvious and the alveo-lar septum was thicker in group A. The change of alveoli in group C was obviously weaker than group A and group B. The RAC of group A and group B were significantly lower than those in the air group(P<0. 05). The RAC of group C was slightly lower than those in the air group,and had statistical significance at 14 d (P<0. 05). The MAD and AST of group A and group B were significantly higher than those in the air group at 7 d (P<0. 05). The MAD and AST of group C decreased slightly over time,and had statistical signifi-cance at 21 d( P<0. 05). Conclusion Different concentrations of oxygen can have an impact on alveolar development. Severe alveolar dysplasia can be seen after continuous inhalation of more than 60% oxygen concentration. It provides a model basis for the study of the pathogenesis of bronchopulmonary dysplasia.
