Grape seed proanthocyanidin extract inhibits platelet-derived growth factor induced proliferation of airway smooth muscle cells
10. 3760/cma. j. issn. 1673-4912. 2019. 07. 004
- VernacularTitle:葡萄籽原青花素对血小板衍生生长因子诱导的大鼠气道平滑肌细胞增殖的影响
- Author:
Tianyue WANG
1
;
Qianlan ZHOU
;
Yunxiao SHANG
Author Information
1. 中国医科大学附属盛京医院小儿呼吸内科
- Keywords:
Grape seed proanthocyanidin extract;
Airway smooth muscle cells;
Proliferation;
Extra-cellular regulatory protein kinase
- From:
Chinese Pediatric Emergency Medicine
2019;26(7):497-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of grape seed proanthocyanidin extract(GSPE) on the proliferation of airway smooth muscle cells( ASMCs) induced by platelet-derived growth factor( PDGF) and the underlying molecular mechanism. Methods ASMCs of primary rat were cultured. MTT and flow cytom-etry were used to detect the cell proliferation activity and cell cycle distribution of ASMCs which were treated with PDGF and GSPE respectively. The expression levels of cyclin D1,extracellular regulated protein kinases ( ERK)1/2,p-ERK1/2 and β-actin protein in each group ASMCs were analyzed using western blotting assay after ERK1/2 inhibitor PD98059 intervention. Results Compared with control group,cell proliferative activ-ity,S phase fraction and the expression of cyclin D1 and p-ERK1/2 protein increased in PDGF induced group (P<0. 05). These effects induced by PDGF could be reversed by GSPE. PD98059 also could block PDGF induced higher expression of p-ERK1/2 and cyclin D1 proteins in rat ASMCs. Conclusion GSPE can inhib-it PDGF induced cell proliferation and via ERK1/2 signaling pathway in rat ASMCs,which provide a new way for treatment of bronchial asthma.
