Evaluation of FilmArray respiratory panel for the rapid and multi￣pathogen detection in children with severe pneumonia
10.3760/cma.j.issn.1673-4912.2019.06.007
- VernacularTitle:呼吸道panel巢式多重聚合酶链反应快速病原体检测在儿童重症肺炎中的作用
- Author:
Fei WANG
1
;
Yun CUI
;
Hong ZHANG
;
Jingyi SHI
;
Yan ZHU
;
Yiping ZHOU
;
Rongxin CHEN
;
Chunxia WANG
;
Yucai ZHANG
Author Information
1. 上海交通大学附属儿童医院重症医学科 200062
- Keywords:
Severe pneumonia;
Respiratory panel (R-Panel);
Multiplex polymerase chain reaction;
Critically ill;
Children
- From:
Chinese Pediatric Emergency Medicine
2019;26(6):427-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of nested polymerase chain reaction (PCR) for the rap-id detection of pathogens in children with severe pneumonia. Methods We prospectively enrolled the pa-tients with severe community-acquired pneumonia admitted to pediatric intensive care unit (PICU) in Shang-hai Children′s Hospital from January 2017 to June 2018. The sputum for PCR were collected within 24 h after PICU admission. Both nested PCR and routine microbiological methods were performed. Respiratory Panel (R-Panel) based on nested PCR could detect 17 kinds of respiratory pathogen at the same time. Results A total of 65 patients were enrolled in this study and the samples were detected using both R-Panel and routine microbiological method. (1) A total of 15 patients (23. 08 %) showed positive routine microbiological de-tection including 13 cases with virus-positive and 2 cases with mycoplasma-positive; (2) A total of 38 pa-tients (58. 46%) showed positive results using R-Panel within 2 h including 46 cases with virus-positive and 5 cases with mycoplasma-positive. The mainly primary infection was human rhinovirus and enterovirus in 15 cases (23. 08%),followed by adenovirus in 10 cases (15. 38%); and the positive rate of 2 or more patho-gens was 18. 46% (12/65);(3) The rate of adenovirus-positive using R-Panel was significantly higher than that using routine microbiological methods (15. 38 % vs. 4. 62%,χ2 ＝4. 188,P＝0. 041); the sensitivity of R-Panel for detection of adenovirus,respiratory syncytial virus,and parainfluenza virus was significantly high-er than those of routine microbiological methods (100% vs. 30%,χ2 ＝107. 692;50. 00% vs. 16. 67%,χ2 ＝ 24. 442;100% vs. 80%,χ2 ＝22. 222;100% vs. 40%,χ2 ＝85. 714; all P <0. 001). Conclusion R-Panel using nested PCR is a rapid,sensitive,and specific method for the detection of pathogens in children with severe community acquired pneumonia,which is valuable for targeted therapy in time.
