Structures and Antigenic Epitopes of Dust Mite Allergens: Der p 2 and Der f 2: a Comparative Study
10.3969/j.issn.1671-332X.2017.11.030
- VernacularTitle:尘螨过敏原蛋白Der p2和Der f 2结构与抗原表位的比较研究
- Author:
Min LIANG
1
;
Xiao FENG
;
La HU
;
Jitao CHEN
;
Ming LI
;
Yuxiong LAI
;
Zhaoyu LIU
Author Information
1. 广州医科大学附属第五医院 广东广州 510700
- Keywords:
Allergen Protein;
Der p 2;
Der f 2;
B Cell Epitope;
HLA High Affinity Site
- From:
Modern Hospital
2017;17(11):1665-1669
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare and analyze the primary and secondary structures and antigenic epitopes of the two allergens: Der p 2 and Der f 2. Methods The protein sequences of Der p 2 and Der f 2 were downloaded online. The primary and secondary structures of the dust mite allergens were compared and analyzed bioinformatically to determine the potential epitope and signal peptide sites. Results Both Der p 2 and Der f 2 contained 146 amino acids and 9 potential protein binding sites with a secondary structure that mainly contains [3 - sheets, and there might be signal peptides site at the 1st 17th segment of the N - terminus. B cell epitopes analysis revealed that both Der p 2 and Der f 2 have 9 potential linear B epitopes and 2 conformational B epitopes. NetMHCⅡserver prediction showed Der p 2 contains 6 high affinity sites, whereas Der f 2 0nly contains 5. Conclusion This study may lay the foundation for further research of the biochemical function of the 2 allergens and contribute to vaccine development for allergen - specific immunotherapy.
