Mechanism study of sanguinarine inhibiting tumor growth of ovarian cancer by apoptosis induction
10. 11904/j. issn. 1002-3070. 2019. 04. 004
- VernacularTitle:血根碱通过诱导细胞凋亡抑制卵巢癌肿瘤生长的机制研究
- Author:
Beilei ZHANG
1
;
Yi LI
;
Tao WU
;
Feng JIANG
;
Hongxi ZHAO
;
Yanhong LI
Author Information
1. 空军军医大学唐都医院妇产科(西安 710038
- Keywords:
Sanguinarine;
Apoptosis;
Reactive oxygen species;
Ovarian cancer
- From:Practical Oncology Journal
2019;33(4):305-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the mechanism of sanguinarine(SANG)on the inhibitory pro-liferation in ovarian cancer SKOV3 cells. Methods CCK-8 assay was used to detect proliferation of SKOV3 cells. Flow cytometry was used to detect the effect of SANG on apoptosis in SKOV3 cells. The spectrophotometer was used to detect the production of reac-tive oxygen species(ROS)by SANG. The mouse ovarian cancer xenograft model was used to detect the inhibitory effect of SANG on tumor growth. Results SANG promoted apoptosis in SKOV3 cells in a dose-and time-dependent manner. The SANG-induced ap-optosis was associated with the production of ROS,Activated the c-Jun-N-terminal kinase( JNK) and nuclear factor-κB( NF-κB)signaling pathways. In mouse model of ovarian cancer xenografts,after intravenous injection of mice with SANG,SANG was signifi-cantly inhibited the growth of ovarian cancer xenografts when compared to the control group. SANG also significantly induced apoptosis in ovarian cancer xenografts. Conclusion SANG can significantly inhibit the proliferation of ovarian cancer SKOV3 cells,induce ap-optosis,increase the production of ROS,and inhibit the growth of ovarian cancer.
