Atractylenolide I regulates proliferation and invasion of lung cancer A549 cells through the TLR4/MyD88 pathway
10.11904/j.issn.1002-3070.2019.03.007
- VernacularTitle:白术内酯Ⅰ通过TLR4/MyD88通路调控肺癌A549细胞增殖侵袭的研究
- Author:
Zhiqiang LIU
1
;
Yanjie CHU
;
Jing LIU
;
Yanbo WANG
Author Information
1. 哈尔滨医科大学附属肿瘤医院血液科(哈尔滨 150081)
- Keywords:
Atratcylenolide I;
Lung cancer A549 cells;
The TLR4/MyD88 pathway;
Proliferation;
Invasion
- From:
Practical Oncology Journal
2019;33(3):228-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the role and molecular mechanism of AtractylenolideⅠin the progression of lung cancer. Methods qRT-qPCR and immunohistochemistry were used to detect the expression of TLR4 and MyD88 at levels of mRNA and protein in lung cancer and adjacent tissues. Transwell and MTT assays were used to detect effects of at-ractylenolide I(100 μM)on the invasion,migration and proliferation of A549 cells. Western blot was also used to detect the effect of atractylenolide I on the expression of TLR4 and MyD88 proteins. Results The expression of TLR4 and MyD88 at levels of mRNA and protein was highly expressed in lung cancer tissues when compared to adjacent tissues(P<0. 05). Compared with the control group,the invasion ability of A549 cells in the Atractylenolide I group was significantly decreased,and the proliferative activity was in-hibited(P<0. 05). Atractylenolide I inhibited the expression of TLR4 and MyD88 protein in A549 cells( P<0. 05). Conclusion
