Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects.
10.5125/jkaoms.2016.42.2.90
- Author:
In Woong UM
1
;
Suk Hyun HWANG
;
Young Kyun KIM
;
Moon Young KIM
;
Sang Ho JUN
;
Jae Jun RYU
;
Hyon Seok JANG
Author Information
1. R&D Institute, Korea Tooth Bank, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Demineralized dentin matrix;
Recombinant human bone morphogenetic protein-2;
Microcomputed tomography;
Histomorphometric analysis
- MeSH:
Dentin*;
Humans*;
Osteogenesis;
Skull;
X-Ray Microtomography
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2016;42(2):90-98
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2. MATERIALS AND METHODS: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (µCT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals. RESULTS: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The µCT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks. CONCLUSION: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
